APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:50000 - 1:200000 |
| 1:50 - 1:1000 |
| 1:50 - 1:1000 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein of hmuan Lamin A/C
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
lamin A/C , CDCD1 , CDDC , CMD1A , CMT2B1 , EMD2 , FPL , FPLD , FPLD2 , HGPS , IDC , LDP1 , LFP , LGMD1B , LMN1 , LMNC , LMNL1 , MADA , PRO1
Cellular Localization
Nucleus,nucleoplasm
Background
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq, Apr 2012]
Database
Research Area
DATA IMAGES
![Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774](/templates/default/js/lightbox/images/loading.gif "Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774")
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GTX00774 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody [GT1137] (GTX00774) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX00774 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX00774 Lamin A + C antibody [GT1137].
Green: Primary antibody
Blue: DAPI
Dlution : 1:200
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GTX00774 WB Image
WB analysis of various samples using GTX00774 Lamin A + C antibody [GT1137].
Dilution : 1:50000
Loading : 25μg
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GTX00774 WB Image
WB analysis of NIH-3T3 extracts using GTX00774 Lamin A + C antibody [GT1137].
WCE : whole cell lysate
CE : cytoplasmic extracts
NE : nuclear extracts
Dilution : 1:10000
Loading : 25μg
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GTX00774 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX00774 Lamin A + C antibody [GT1137].
Red: Primary antibody
Blue: DAPI
Detection system: confocol
Dlution : 1:100
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GTX00774 WB Image
WB analysis of HeLa whole cell lysate using GTX00774 Lamin A + C antibody [GT1137].
Dilution :1:10000-1:2560000
Loading : 25μg
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GTX00774 IHC-P Image
IHC-P analysis of hmuan breast cancer tissue section using GTX00774 Lamin A + C antibody [GT1137].
Dlution : 1:200
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GTX00774 IP Image
IP analysis of K562 whole cell lysate using GTX00774 Lamin A + C antibody [GT1137].
Total protein amount : 200μg
IP antibody : 3μg
Dilution : 1:20000
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GTX00774 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX00774 Lamin A + C antibody [GT1137].
Pink: Primary antibody
Blue: DAPI
Dlution : 1:100-1:1600
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GTX00774 ICC/IF Image
ICC/IF analysis of L929 cells using GTX00774 Lamin A + C antibody [GT1137].
Green: Primary antibody
Blue: DAPI
The image was taken by Confocal microscopy.
Dlution : 1:200
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GTX00774 ICC/IF Image
ICC/IF analysis of H9C2 cells using GTX00774 Lamin A + C antibody [GT1137].
Green: Primary antibody
Blue: DAPI
Dlution : 1:200
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GTX00774 WB Image
WB analysis of C6 extracts using GTX00774 Lamin A + C antibody [GT1137].
WCE : whole cell lysate
CE : cytoplasmic extracts
NE : nuclear extracts
Dilution : 1:10000
Loading : 25μg
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GTX00774 WB Image
WB analysis of HeLa extracts using GTX00774 Lamin A + C antibody [GT1137].
WCE : whole cell lysate
CE : cytoplasmic extracts
NE : nuclear extracts
Dilution : 1:10000
Loading : 25μg
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GTX00774 IHC-P Image
IHC-P analysis of hmuan esophageal cancer tissue section using GTX00774 Lamin A + C antibody [GT1137].
Dlution : 1:400 - 1:3200
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GTX00774 WB Image
WB analysis of Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts using GTX00774 Lamin A + C antibody [GT1137].
Dilution : 1:50000
Loading : 25μg
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REFERENCE
There are currently no references for Lamin A + C antibody [GT1137] (GTX00774). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Lamin A + C antibody [GT1137] (GTX00774). Be the first to share your experience with this product.
![Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774](/upload/website/prouct_img/small/GTX00774/GTX00774_3560448007_20200313_WB_w_23053121_414.webp "Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774](/upload/website/prouct_img/small/GTX00774/GTX00774_20191101_AP_002_18_w_23053121_131.webp "Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774](/upload/website/prouct_img/small/GTX00774/GTX00774_20200327_WB_2_w_23053121_343.webp "Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774](/upload/website/prouct_img/small/GTX00774/GTX00774_20200327_WB_4_w_23053121_568.webp "Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774](/upload/website/prouct_img/small/GTX00774/GTX00774_20191101_AP_002_20_w_23053121_867.webp "Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774](/upload/website/prouct_img/small/GTX00774/GTX00774_20200327_WB_1_w_23053121_865.webp "Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in IHC (Paraffin sections) (IHC-P). GTX00774](/upload/website/prouct_img/small/GTX00774/GTX00774_20191101_AP_003_15_w_23053121_217.webp "Anti-Lamin A + C antibody [GT1137] used in IHC (Paraffin sections) (IHC-P). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in Immunoprecipitation (IP). GTX00774](/upload/website/prouct_img/small/GTX00774/GTX00774_20200327_IP_7_w_23053121_725.webp "Anti-Lamin A + C antibody [GT1137] used in Immunoprecipitation (IP). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774](/upload/website/prouct_img/small/GTX00774/GTX00774_20191101_AP_002_21_w_23053121_755.webp "Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774](/upload/website/prouct_img/small/GTX00774/GTX00774_20191101_AP_002_19_w_23053121_348.webp "Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774](/upload/website/prouct_img/small/GTX00774/GTX00774_20191101_AP_002_17_w_23053121_128.webp "Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774](/upload/website/prouct_img/small/GTX00774/GTX00774_20200327_WB_5_w_23053121_251.webp "Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774](/upload/website/prouct_img/small/GTX00774/GTX00774_20200327_WB_3_w_23053121_355.webp "Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in IHC (Paraffin sections) (IHC-P). GTX00774](/upload/website/prouct_img/small/GTX00774/GTX00774_20191101_AP_003_16_w_23053121_287.webp "Anti-Lamin A + C antibody [GT1137] used in IHC (Paraffin sections) (IHC-P). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774](/upload/website/prouct_img/small/GTX00774/GTX00774_20200327_WB_6_w_23053121_568.webp "Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774](/upload/website/prouct_img/normal/GTX00774/GTX00774_3560448007_20200313_WB_w_23053121_414.webp "Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774](/upload/website/prouct_img/normal/GTX00774/GTX00774_20191101_AP_002_18_w_23053121_131.webp "Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774](/upload/website/prouct_img/normal/GTX00774/GTX00774_20200327_WB_2_w_23053121_343.webp "Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774](/upload/website/prouct_img/normal/GTX00774/GTX00774_20200327_WB_4_w_23053121_568.webp "Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774](/upload/website/prouct_img/normal/GTX00774/GTX00774_20191101_AP_002_20_w_23053121_867.webp "Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774](/upload/website/prouct_img/normal/GTX00774/GTX00774_20200327_WB_1_w_23053121_865.webp "Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in IHC (Paraffin sections) (IHC-P). GTX00774](/upload/website/prouct_img/normal/GTX00774/GTX00774_20191101_AP_003_15_w_23053121_217.webp "Anti-Lamin A + C antibody [GT1137] used in IHC (Paraffin sections) (IHC-P). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in Immunoprecipitation (IP). GTX00774](/upload/website/prouct_img/normal/GTX00774/GTX00774_20200327_IP_7_w_23053121_725.webp "Anti-Lamin A + C antibody [GT1137] used in Immunoprecipitation (IP). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774](/upload/website/prouct_img/normal/GTX00774/GTX00774_20191101_AP_002_21_w_23053121_755.webp "Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774](/upload/website/prouct_img/normal/GTX00774/GTX00774_20191101_AP_002_19_w_23053121_348.webp "Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774](/upload/website/prouct_img/normal/GTX00774/GTX00774_20191101_AP_002_17_w_23053121_128.webp "Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774](/upload/website/prouct_img/normal/GTX00774/GTX00774_20200327_WB_5_w_23053121_251.webp "Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774](/upload/website/prouct_img/normal/GTX00774/GTX00774_20200327_WB_3_w_23053121_355.webp "Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in IHC (Paraffin sections) (IHC-P). GTX00774](/upload/website/prouct_img/normal/GTX00774/GTX00774_20191101_AP_003_16_w_23053121_287.webp "Anti-Lamin A + C antibody [GT1137] used in IHC (Paraffin sections) (IHC-P). GTX00774")
![Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774](/upload/website/prouct_img/normal/GTX00774/GTX00774_20200327_WB_6_w_23053121_568.webp "Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774")