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Lamin A + C antibody [GT1137]

Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774
Anti-Lamin A + C antibody [GT1137] used in IHC (Paraffin sections) (IHC-P). GTX00774
Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774
Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774
Anti-Lamin A + C antibody [GT1137] used in IHC (Paraffin sections) (IHC-P). GTX00774
Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774
Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774
Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774
Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774
Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774
Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774
Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774
Anti-Lamin A + C antibody [GT1137] used in Immunoprecipitation (IP). GTX00774
Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774
Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774

Cat No. GTX00774

Host

Rabbit

Clonality

Monoclonal

Clone Name

GT1137

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:50000-1:200000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
IP 1:50-1:200
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (pH 7.3) with 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein of hmuan Lamin A/C

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lamin A/C , CDCD1 , CDDC , CMD1A , CMT2B1 , EMD2 , FPL , FPLD , FPLD2 , HGPS , IDC , LDP1 , LFP , LGMD1B , LMN1 , LMNC , LMNL1 , MADA , PRO1

Cellular Localization

Nucleus,nucleoplasm

Background

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq, Apr 2012]

Database

Research Area

DATA IMAGES

Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774

GTX00774 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody [GT1137] (GTX00774) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Lamin A + C antibody [GT1137] used in IHC (Paraffin sections) (IHC-P). GTX00774

GTX00774 IHC-P Image

IHC-P analysis of hmuan breast cancer tissue section using GTX00774 Lamin A + C antibody [GT1137].
Dlution : 1:200

Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774

GTX00774 ICC/IF Image

ICC/IF analysis of H9C2 cells using GTX00774 Lamin A + C antibody [GT1137].
Green: Primary antibody
Blue: DAPI
Dlution : 1:200

Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774

GTX00774 ICC/IF Image

ICC/IF analysis of L929 cells using GTX00774 Lamin A + C antibody [GT1137].
Green: Primary antibody
Blue: DAPI
The image was taken by Confocal microscopy.
Dlution : 1:200

Anti-Lamin A + C antibody [GT1137] used in IHC (Paraffin sections) (IHC-P). GTX00774

GTX00774 IHC-P Image

IHC-P analysis of hmuan esophageal cancer tissue section using GTX00774 Lamin A + C antibody [GT1137].
Dlution : 1:400 - 1:3200

Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774

GTX00774 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX00774 Lamin A + C antibody [GT1137].
Green: Primary antibody
Blue: DAPI
Dlution : 1:200

Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774

GTX00774 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00774 Lamin A + C antibody [GT1137].
Red: Primary antibody
Blue: DAPI
Detection system: confocol
Dlution : 1:100

Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774

GTX00774 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX00774 Lamin A + C antibody [GT1137].
Pink: Primary antibody
Blue: DAPI
Dlution : 1:100-1:1600

Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774

GTX00774 WB Image

WB analysis of various samples using GTX00774 Lamin A + C antibody [GT1137].
Dilution : 1:50000
Loading : 25μg

Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774

GTX00774 WB Image

WB analysis of HeLa extracts using GTX00774 Lamin A + C antibody [GT1137].
WCE : whole cell lysate
CE : cytoplasmic extracts
NE : nuclear extracts
Dilution : 1:10000
Loading : 25μg

Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774

GTX00774 WB Image

WB analysis of Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts using GTX00774 Lamin A + C antibody [GT1137].
Dilution : 1:50000
Loading : 25μg

Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774

GTX00774 WB Image

WB analysis of NIH-3T3 extracts using GTX00774 Lamin A + C antibody [GT1137].
WCE : whole cell lysate
CE : cytoplasmic extracts
NE : nuclear extracts
Dilution : 1:10000
Loading : 25μg

Anti-Lamin A + C antibody [GT1137] used in Immunoprecipitation (IP). GTX00774

GTX00774 IP Image

IP analysis of K562 whole cell lysate using GTX00774 Lamin A + C antibody [GT1137].
Total protein amount : 200μg
IP antibody : 3μg
Dilution : 1:20000

Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774

GTX00774 WB Image

WB analysis of C6 extracts using GTX00774 Lamin A + C antibody [GT1137].
WCE : whole cell lysate
CE : cytoplasmic extracts
NE : nuclear extracts
Dilution : 1:10000
Loading : 25μg

Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774

GTX00774 WB Image

WB analysis of HeLa whole cell lysate using GTX00774 Lamin A + C antibody [GT1137].
Dilution :1:10000-1:2560000
Loading : 25μg

REFERENCE

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REVIEW

There are currently no reviews for Lamin A + C antibody [GT1137] (GTX00774). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf