GeneTex
  • Country / Location Selection

United States (US)

Lamin A + C antibody [GT9712]

Anti-Lamin A + C antibody [GT9712] used in Western Blot (WB). GTX629404
Anti-Lamin A + C antibody [GT9712] used in Western Blot (WB). GTX629404
Anti-Lamin A + C antibody [GT9712] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629404
Anti-Lamin A + C antibody [GT9712] used in Immunoprecipitation (IP). GTX629404

Cat No. GTX629404

Host

Mouse

Clonality

Monoclonal

Clone Name

GT9712

Isotype

IgG2b

Application

WB, ICC/IF, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

HeLa , HeLa nuclear

Predict Reactivity

Mouse, Rat, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lamin A + C. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888110

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lamin A/C , CDCD1 , CDDC , CMD1A , CMT2B1 , EMD2 , FPL , FPLD , FPLD2 , HGPS , IDC , LDP1 , LFP , LGMD1B , LMN1 , LMNC , LMNL1 , MADA , PRO1

Cellular Localization

Nucleus

Background

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Through alternate splicing, this gene encodes three type A lamin isoforms. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Lamin A + C antibody [GT9712] used in Western Blot (WB). GTX629404

GTX629404 WB Image

Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody [GT9712] (GTX629404) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Lamin A + C antibody [GT9712] used in Western Blot (WB). GTX629404

GTX629404 WB Image

Lamin A + C antibody [GT9712] detects Lamin A + C protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg HeLa nuclear lysate/extract
7.5 % SDS-PAGE
Lamin A + C antibody [GT9712] (GTX629404) dilution: 1:1000

Anti-Lamin A + C antibody [GT9712] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629404

GTX629404 ICC/IF Image

Lamin A + C antibody detects Lamin A + C protein at nuclear envelope by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20ºC 100% methanol for 5 min.
Green: Lamin A + C protein stained by Lamin A + C antibody (GTX629404) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Lamin A + C antibody [GT9712] used in Immunoprecipitation (IP). GTX629404

GTX629404 IP Image

Immunoprecipitation of Lamin A + C protein from HeLa whole cell extracts using 5 μg of Lamin A + C antibody [GT9712] (GTX629404).
Western blot analysis was performed using Lamin A + C antibody [GT9712] (GTX629404).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

REFERENCE

There are currently no references for Lamin A + C antibody [GT9712] (GTX629404). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Lamin A + C antibody [GT9712] (GTX629404). Be the first to share your experience with this product.
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169
Custom Conjugations