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Lamin A + C antibody [mab636]

Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813
Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813
Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813
Anti-Lamin A + C antibody [mab636] used in Western Blot (WB). GTX80813
Anti-Lamin A + C antibody [mab636] used in Western Blot (WB). GTX80813
Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813
Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813
Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813
Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813

Cat No. GTX80813

Host

Mouse

Clonality

Monoclonal

Clone Name

mab636

Isotype

IgG2b

Application

WB, ICC/IF, IHC-Fr

Reactivity

Human, Mouse, Bovine, Dog, Pig
Package
200 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100 - 1:2000
ICC/IF 1:100
IHC-Fr 1:100
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Pig

Immunogen

Porcine lamin preparation.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lamin A/C , CDCD1 , CDDC , CMD1A , CMT2B1 , EMD2 , FPL , FPLD , FPLD2 , HGPS , IDC , LDP1 , LFP , LGMD1B , LMN1 , LMNC , LMNL1 , MADA , PRO1

Cellular Localization

Nucleus,nucleoplasm

Background

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq, Apr 2012]

Database

Research Area

DATA IMAGES

Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813

GTX80813 ICC/IF Image

ICC/IF analysis of HMVEC Cells using GTX80813 Lamin A + C antibody [mab636].
Green : Primary antibody
Blue : Nuclei

Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813

GTX80813 ICC/IF Image

ICC/IF analysis of Human fibroblast cells using GTX80813 Lamin A + C antibody [mab636].
Fixation : methanol
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:200 for at least 1 hour at room temperature

Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813

GTX80813 ICC/IF Image

ICC/IF analysis of U251 cells using GTX80813 Lamin A + C antibody [mab636]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-Lamin A + C antibody [mab636] used in Western Blot (WB). GTX80813

GTX80813 WB Image

WB analysis of 10μg nuclear extract from fractionated mouse tissue lysate using GTX80813 Lamin A + C antibody [mab636].
Dilution : 1:500

Anti-Lamin A + C antibody [mab636] used in Western Blot (WB). GTX80813

GTX80813 WB Image

WB analysis of various amounts of human fibroblast whole cell lysate using GTX80813 Lamin A + C antibody [mab636].
Dilution : 1:2000

Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813

GTX80813 ICC/IF Image

ICC/IF analysis of MDCK cells using GTX80813 Lamin A + C antibody [mab636].
Red : Primary antibody
Blue : cell nucleus
Permeabilization : 0.1% Triton X-100
Dilution : 1:100 in blocking buffer for at least 1 hour at room temperature

Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813

GTX80813 ICC/IF Image

ICC/IF analysis of A549 cells using GTX80813 Lamin A + C antibody [mab636].
Green : Primary antibody
Blue : Nuclei

Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813

GTX80813 ICC/IF Image

ICC/IF analysis of U2-OS cells (A) or HeLa cells (B) using GTX80813 Lamin A + C antibody [mab636].
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813

GTX80813 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX80813 Lamin A + C antibody [mab636].

REFERENCE

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REVIEW

There are currently no reviews for Lamin A + C antibody [mab636] (GTX80813). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf