GeneTex
United States (US)

Lamin A + C antibody

KO/KD
GTX101126_40156_IHC_M_2_19011506_826.jpg
GTX101126_40156_20170323_WB_KO_watermark_19011506_157.jpg
GTX101126_40051_WB_M_19011506_162.jpg
GTX101126_40156_20151105_WB_M_19011506_634.jpg
GTX101126_40156_IFA_19011506_385.jpg
GTX101126_40156_20151106_IP_19011506_235.jpg
GTX101126_40156_IHC_M_3_19011506_181.jpg
GTX101126_40156_IHC_M_19011506_335.jpg
GTX101126_40156_IHC_19011506_655.jpg
GTX101126_40156_IHC_3_19011506_617.jpg
GTX101126_40156_IHC_2_19011506_819.jpg

Cat No. GTX101126

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:66-1:500
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

HeLa , NIH-3T3 , C2C12

Predict Reactivity

Rat, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lamin A/C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Lamin A/C,Cdcd1,Cddc,Cmd1A,Cmt2B1,Emd2,Fpl,Fpld,Fpld2,Hgps,Idc,Ldp1,Lfp,Lgmd1B,Lmn1,Lmnc,Lmnl1,Mada,Pro1,Lmna

Cellular Localization

Nucleus

Background

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq, Apr 2012]

Database

Research Area

DATA IMAGES
GTX101126_40156_IHC_M_2_19011506_826.jpg

GTX101126 IHC-P Image

Lamin A + C antibody detects Lamin A + C protein at nuclear envelope on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Lamin A + C antibody (GTX101126) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101126_40156_20170323_WB_KO_watermark_19011506_157.jpg

GTX101126 WB Image

KO/KD

Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (GTX101126) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CRISPR knockout cell lines generated by www.edigene.com

GTX101126_40051_WB_M_19011506_162.jpg

GTX101126 WB Image

Lamin A + C antibody detects Lamin A + C protein by Western blot analysis.
A. 30 μg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
Lamin A + C antibody (GTX101126) dilution: 1:1000

GTX101126_40156_20151105_WB_M_19011506_634.jpg

GTX101126 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (GTX101126) diluted at 1:1000.

GTX101126_40156_IFA_19011506_385.jpg

GTX101126 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Lamin A + C (GTX101126) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

GTX101126_40156_20151106_IP_19011506_235.jpg

GTX101126 IP Image

Immunoprecipitation of Lamin A + C protein from HeLa whole cell extracts using 5 μg of Lamin A + C antibody (GTX101126).
Western blot analysis was performed using Lamin A + C antibody (GTX101126).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101126_40156_IHC_M_3_19011506_181.jpg

GTX101126 IHC-P Image

Lamin A + C antibody detects Lamin A + C protein at nuclear envelope on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Lamin A + C antibody (GTX101126) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101126_40156_IHC_M_19011506_335.jpg

GTX101126 IHC-P Image

Lamin A + C antibody detects Lamin A + C protein at nuclear envelope on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
Lamin A + C antibody (GTX101126) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101126_40156_IHC_19011506_655.jpg

GTX101126 IHC-P Image

Lamin A + C antibody detects Lamin A + C protein at membrane on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Lamin A + C antibody (GTX101126) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101126_40156_IHC_3_19011506_617.jpg

GTX101126 IHC-P Image

Lamin A + C antibody detects Lamin A + C protein at nuclear envelope on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
Lamin A + C antibody (GTX101126) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101126_40156_IHC_2_19011506_819.jpg

GTX101126 IHC-P Image

Lamin A + C antibody detects Lamin A + C protein at nuclear envelope on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Lamin A + C antibody (GTX101126) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169