APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:66-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
C2C12 , NIH-3T3 , HeLa
Predict Reactivity
Rat, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.27 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Lamin A + C. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10617300
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
lamin A/C , CDCD1 , CDDC , CMD1A , CMT2B1 , EMD2 , FPL , FPLD , FPLD2 , HGPS , IDC , LDP1 , LFP , LGMD1B , LMN1 , LMNC , LMNL1 , MADA , PRO1
Cellular Localization
Nucleus
Background
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq, Apr 2012]
Database
Research Area
DATA IMAGES
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GTX101126 IHC-P Image
Lamin A + C antibody detects Lamin A + C protein at nuclear envelope on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
Lamin A + C antibody (GTX101126) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101126 IHC-P Image
Lamin A + C antibody detects Lamin A + C protein at nuclear envelope on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
Lamin A + C antibody (GTX101126) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101126 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (GTX101126) diluted at 1:1000.
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GTX101126 IHC-P Image
Lamin A + C antibody detects Lamin A + C protein at membrane on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Lamin A + C antibody (GTX101126) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101126 IHC-P Image
Lamin A + C antibody detects Lamin A + C protein at nuclear envelope on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Lamin A + C antibody (GTX101126) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101126 WB Image
Lamin A + C antibody detects Lamin A + C protein by Western blot analysis.
A. 30 μg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
Lamin A + C antibody (GTX101126) dilution: 1:1000
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GTX101126 IHC-P Image
Lamin A + C antibody detects Lamin A + C protein at nuclear envelope on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Lamin A + C antibody (GTX101126) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101126 WB Image
Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (GTX101126) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX101126 IHC-P Image
Lamin A + C antibody detects Lamin A + C protein at nuclear envelope on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Lamin A + C antibody (GTX101126) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101126 IP Image
Immunoprecipitation of Lamin A + C protein from HeLa whole cell extracts using 5 μg of Lamin A + C antibody (GTX101126).
Western blot analysis was performed using Lamin A + C antibody (GTX101126).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX101126 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Lamin A + C (GTX101126) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.
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REFERENCE
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