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Lamin A + C antibody

Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX111677
Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX111677
Anti-Lamin A + C antibody used in Western Blot (WB). GTX111677
Anti-Lamin A + C antibody used in Western Blot (WB). GTX111677
Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX111677

Cat No. GTX111677

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

HeLa Nucleus , A431 , NIH-3T3

Predict Reactivity

Rat, Bovine, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lamin A + C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950819

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lamin A/C , CDCD1 , CDDC , CMD1A , CMT2B1 , EMD2 , FPL , FPLD , FPLD2 , HGPS , IDC , LDP1 , LFP , LGMD1B , LMN1 , LMNC , LMNL1 , MADA , PRO1

Cellular Localization

Nucleus

Background

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Through alternate splicing, this gene encodes three type A lamin isoforms. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX111677

GTX111677 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 Xenograft, using Lamin A + C(GTX111677) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX111677

GTX111677 IHC-P Image

Lamin A + C antibody detects Lamin A + C protein at nuclear envelope on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Lamin A + C antibody (GTX111677) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Lamin A + C antibody used in Western Blot (WB). GTX111677

GTX111677 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (GTX111677) diluted at 1:1000.

Anti-Lamin A + C antibody used in Western Blot (WB). GTX111677

GTX111677 WB Image

Sample (20 ug )
A: HeLa Nucleus
10% SDS PAGE
GTX111677 diluted at 1:3000

Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX111677

GTX111677 IHC-P Image

Lamin A + C antibody detects Lamin A + C protein at nuclear envelope on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Lamin A + C antibody (GTX111677) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169