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Lamin A + C antibody

Anti-Lamin A + C antibody used in Western Blot (WB). GTX13910
Anti-Lamin A + C antibody used in Flow cytometry (FACS). GTX13910
Anti-Lamin A + C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX13910

Cat. No. GTX13910

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, FACS

Reactivity

Human, Mouse, Rat, Bovine, Hamster, Pig, Bat, Ferret, Mink, Primate
Package
200 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100
FACS 1:500 - 1:1000
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Product Note

Because of immunogen's 100% homology with Isoforms 1, 4, 5, 6, ADelta10 and C of Prelamin-A/C, this antibody is expected to detect multiple bands in WB.

PROPERTIES

Form

Liquid

Buffer

Serum diluted with PBS, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to amino acids 249-265 CRAQHEDQVEQYKKELEK of Human Lamin A/C was used immunogen.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lamin A/C , CDCD1 , CDDC , CMD1A , CMT2B1 , EMD2 , FPL , FPLD , FPLD2 , HGPS , IDC , LDP1 , LFP , LGMD1B , LMN1 , LMNC , LMNL1 , MADA , PRO1

Cellular Localization

Nucleus,nucleoplasm

Background

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Through alternate splicing, this gene encodes three type A lamin isoforms. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Lamin A + C antibody used in Western Blot (WB). GTX13910

GTX13910 WB Image

WB analysis of HeLa cell lysate using GTX13910 Lamin A + C antibody.
Dilution : 1:1000
Loading : 15μg

Anti-Lamin A + C antibody used in Flow cytometry (FACS). GTX13910

GTX13910 FACS Image

FACS (Intracellular staining) analysis of 10⁶ HeLa cells using GTX13910 Lamin A + C antibody.
Orange : Primary antibody
Blue : secondary antibody control
Dilution : 1:1000

Anti-Lamin A + C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX13910

GTX13910 ICC/IF Image

ICC/IF analysis of A431 cells using GTX13910 Lamin A + C antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399