APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:1000 |
| 1:100 |
| 1:500 - 1:1000 |
Not tested in other applications.
Calculated MW
Product Note
Because of immunogen's 100% homology with Isoforms 1, 4, 5, 6, ADelta10 and C of Prelamin-A/C, this antibody is expected to detect multiple bands in WB.
PROPERTIES
Form
Liquid
Buffer
Serum diluted with PBS, 50% Glycerol
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to amino acids 249-265 CRAQHEDQVEQYKKELEK of Human Lamin A/C was used immunogen.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
lamin A/C , CDCD1 , CDDC , CMD1A , CMT2B1 , EMD2 , FPL , FPLD , FPLD2 , HGPS , IDC , LDP1 , LFP , LGMD1B , LMN1 , LMNC , LMNL1 , MADA , PRO1
Cellular Localization
Nucleus,nucleoplasm
Background
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Through alternate splicing, this gene encodes three type A lamin isoforms. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX13910 ICC/IF Image
ICC/IF analysis of A431 cells using GTX13910 Lamin A + C antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:100
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GTX13910 FACS Image
FACS (Intracellular staining) analysis of 10⁶ HeLa cells using GTX13910 Lamin A + C antibody.
Orange : Primary antibody
Blue : secondary antibody control
Dilution : 1:1000
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GTX13910 WB Image
WB analysis of HeLa cell lysate using GTX13910 Lamin A + C antibody.
Dilution : 1:1000
Loading : 15μg
|
REFERENCE
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REVIEW
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