GeneTex
United States (US)

Lamin A (cleaved Asp230) antibody

Lamin-A-cleaved-Asp230-antibody__GTX86918-2_18121411_281.jpg
Lamin-A-cleaved-Asp230-antibody__GTX86918-1_18121411_273.jpg

Cat No. GTX86918

Host Rabbit
Clonality Polyclonal
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:40000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

50 kDa. ( Note )

Specificity/Sensitivity

Lamin A (Cleaved-Asp230) Antibody detects endogenous levels of fragment of activated Lamin A resulting from cleavage adjacent to Asp230.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Lamin A.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Lamin A/C,Cdcd1,Cddc,Cmd1A,Cmt2B1,Emd2,Fpl,Fpld,Fpld2,Hgps,Idc,Ldp1,Lfp,Lgmd1B,Lmn1,Lmnc,Lmnl1,Mada,Pro1,Lmna

Cellular Localization

Farnesylation of prelamin-A/C facilitates nuclear envelope targeting and subsequent cleaveage by ZMPSTE24/FACE1 to remove the farnesyl group produces mature lamin-A/C, which can then be inserted into the nuclear lamina. EMD is required for proper localiza

Background

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Play an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Prelamin-A/C can accelerate smooth muscle cell senescence. It acts to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence.

Database

Research Area

DATA IMAGES
Lamin-A-cleaved-Asp230-antibody__GTX86918-2_18121411_281.jpg

GTX86918 WB Image

Western blot analysis of extracts from NIH-3T3 cells, treated with Etoposide 25uM 60', using Lamin A (Cleaved-Asp230) antibody (GTX86918). The lane on the right is treated with the synthesized peptide.

Lamin-A-cleaved-Asp230-antibody__GTX86918-1_18121411_273.jpg

GTX86918 IHC-P Image

Immunohistochemistry of paraffin-embedded human lung carcinoma tissue, using Lamin A (Cleaved-Asp230) antibody (GTX86918). The picture on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339