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Lamin B1 antibody

Anti-Lamin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03695
Anti-Lamin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03695
Anti-Lamin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03695
Anti-Lamin B1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX03695
Anti-Lamin B1 antibody used in Flow cytometry (FACS). GTX03695
Anti-Lamin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03695
Anti-Lamin B1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX03695
Anti-Lamin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX03695
Anti-Lamin B1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX03695
Anti-Lamin B1 antibody used in Western Blot (WB). GTX03695
Anti-Lamin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX03695
Anti-Lamin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03695
Anti-Lamin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX03695

Cat. No. GTX03695

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μg ($449)
See all Lamin B1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
ICC/IF 2μg/ml
IHC-P 0.5-1μg/ml
IHC-Fr 0.5-1μg/ml
FACS 1-3μg/1x106 cells

Note :

IHC-P
By Heat
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

5mg BSA, 0.9mg NaCl, 0.2mg Na₂HPO₄

Preservative

0.05mg sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived human Lamin B1 recombinant protein (Position: Q266-C583).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lamin B1 , ADLD , LMN , LMN2 , LMNB

Background

This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-Lamin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03695

GTX03695 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX03695 Lamin B1 antibody. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins.
Dilution : 2 μg/mL

Anti-Lamin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03695

GTX03695 ICC/IF Image

ICC/IF analysis of A549 cells using GTX03695 Lamin B1 antibody.
Dilution : 1 μg/ml
Antigen retrieval : Enzyme antigen retrieval reagent

Anti-Lamin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03695

GTX03695 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX03695 Lamin B1 antibody.
Dilution : 1 μg/ml
Antigen retrieval : Enzyme antigen retrieval reagent

Anti-Lamin B1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX03695

GTX03695 IHC-Fr Image

IHC-Fr analysis of human placenta tissue using GTX03695 Lamin B1 antibody.
Dilution : 1 μg/ml

Anti-Lamin B1 antibody used in Flow cytometry (FACS). GTX03695

GTX03695 FACS Image

FACS analysis of U937 cells using GTX03695 Lamin B1 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample
Antibody amount : 1 μg/1x10⁶ cells

Anti-Lamin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03695

GTX03695 ICC/IF Image

ICC/IF analysis of SMMC-7721 cells using GTX03695 Lamin B1 antibody.
Dilution : 1 μg/ml
Antigen retrieval : Enzyme antigen retrieval reagent

Anti-Lamin B1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX03695

GTX03695 IHC-Fr Image

IHC-Fr analysis of mouse small intestine tissue using GTX03695 Lamin B1 antibody.
Dilution : 1 μg/ml

Anti-Lamin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX03695

GTX03695 IHC-P Image

IHC-P analysis of human mammary cancer tissue using GTX03695 Lamin B1 antibody.
Dilution : 1 μg/ml
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins

Anti-Lamin B1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX03695

GTX03695 IHC-Fr Image

IHC-Fr analysis of rat small intestine tissue using GTX03695 Lamin B1 antibody.
Dilution : 1 μg/ml

Anti-Lamin B1 antibody used in Western Blot (WB). GTX03695

GTX03695 WB Image

WB analysis of various cell lysates using GTX03695 Lamin B1 antibody.
Lane 1 : U87 cell lysate
Lane 2 : PC-12 cell lysate
Lane 3 : NIH-3T3
Dilution: 0.5 μg/mL
Loading: 50 μg per lane

Anti-Lamin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX03695

GTX03695 IHC-P Image

IHC-P analysis of rat intestine tissue using GTX03695 Lamin B1 antibody.
Dilution : 1 μg/ml
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins

Anti-Lamin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03695

GTX03695 ICC/IF Image

ICC/IF analysis of SW480 cells using GTX03695 Lamin B1 antibody.
Dilution : 1 μg/ml
Antigen retrieval : Enzyme antigen retrieval reagent

Anti-Lamin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX03695

GTX03695 IHC-P Image

IHC-P analysis of mouse intestine tissue using GTX03695 Lamin B1 antibody.
Dilution : 1 μg/ml
Antigen retrieval : Heat mediated citrate buffer

REFERENCE

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REVIEW

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Package List Price ($)
$ 449