Lamin B1 antibody

Name: Lamin B1 antibody
Brand: GeneTex
SKU: GTX03695
Price: 449.00 USD

Anti-Lamin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03695

Anti-Lamin B1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX03695

Anti-Lamin B1 antibody used in Flow cytometry (FACS). GTX03695

Anti-Lamin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX03695

Anti-Lamin B1 antibody used in Western Blot (WB). GTX03695

Cat. No. GTX03695

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS
Reactivity	Human, Mouse, Rat
Package	100 μg ($449)

See all Lamin B1 products

Datasheet

View product citations for antibody GTX03695 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.1-0.5μg/ml
ICC/IF	2μg/ml
IHC-P	0.5-1μg/ml
IHC-Fr	0.5-1μg/ml
FACS	1-3μg/1x106 cells
Note : IHC-P By Heat

Not tested in other applications.

Calculated MW

66 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4

Preservative

0.05mg sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived human Lamin B1 recombinant protein (Position: Q266-C583).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lamin B1 , ADLD , LMN , LMN2 , LMNB

Background

This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Database

Gene ID: 4001 LMNB1
UniProt: P20700 LMNB1

Research Area

DATA IMAGES

GTX03695 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX03695 Lamin B1 antibody. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins.
Dilution : 2 μg/mL

GTX03695 ICC/IF Image

ICC/IF analysis of A549 cells using GTX03695 Lamin B1 antibody.
Dilution : 1 μg/ml
Antigen retrieval : Enzyme antigen retrieval reagent

GTX03695 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX03695 Lamin B1 antibody.
Dilution : 1 μg/ml
Antigen retrieval : Enzyme antigen retrieval reagent

GTX03695 IHC-Fr Image

IHC-Fr analysis of human placenta tissue using GTX03695 Lamin B1 antibody.
Dilution : 1 μg/ml

GTX03695 FACS Image

FACS analysis of U937 cells using GTX03695 Lamin B1 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample
Antibody amount : 1 μg/1x10⁶ cells

GTX03695 ICC/IF Image

ICC/IF analysis of SMMC-7721 cells using GTX03695 Lamin B1 antibody.
Dilution : 1 μg/ml
Antigen retrieval : Enzyme antigen retrieval reagent

GTX03695 IHC-Fr Image

IHC-Fr analysis of mouse small intestine tissue using GTX03695 Lamin B1 antibody.
Dilution : 1 μg/ml

GTX03695 IHC-P Image

IHC-P analysis of human mammary cancer tissue using GTX03695 Lamin B1 antibody.
Dilution : 1 μg/ml
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins

GTX03695 IHC-Fr Image

IHC-Fr analysis of rat small intestine tissue using GTX03695 Lamin B1 antibody.
Dilution : 1 μg/ml

GTX03695 WB Image

WB analysis of various cell lysates using GTX03695 Lamin B1 antibody.
Lane 1 : U87 cell lysate
Lane 2 : PC-12 cell lysate
Lane 3 : NIH-3T3
Dilution: 0.5 μg/mL
Loading: 50 μg per lane

GTX03695 IHC-P Image

IHC-P analysis of rat intestine tissue using GTX03695 Lamin B1 antibody.
Dilution : 1 μg/ml
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins

GTX03695 ICC/IF Image

ICC/IF analysis of SW480 cells using GTX03695 Lamin B1 antibody.
Dilution : 1 μg/ml
Antigen retrieval : Enzyme antigen retrieval reagent

GTX03695 IHC-P Image

IHC-P analysis of mouse intestine tissue using GTX03695 Lamin B1 antibody.
Dilution : 1 μg/ml
Antigen retrieval : Heat mediated citrate buffer

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 449

Qty

Lamin B1 antibody

Cat. No. GTX03695

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Background

Database

Research Area

DATA IMAGES

GTX03695 ICC/IF Image

GTX03695 ICC/IF Image

GTX03695 ICC/IF Image

GTX03695 IHC-Fr Image

GTX03695 FACS Image

GTX03695 ICC/IF Image

GTX03695 IHC-Fr Image

GTX03695 IHC-P Image

GTX03695 IHC-Fr Image

GTX03695 WB Image

GTX03695 IHC-P Image

GTX03695 ICC/IF Image

GTX03695 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.