GeneTex
United States (US)

Lamin B1 antibody

KO/KD
GTX103292_39967_20170601_WB_KO_watermark_19011506_200.jpg
GTX103292_39967_IHC_19011506_179.jpg
GTX103292_39967_20150522_IP_19011506_174.jpg
GTX103292_39967_WB_19011506_237.jpg

Cat No. GTX103292

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP
Reactivity Human, Hamster
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , *Huh7

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lamin B1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ADLD antibody, LMN antibody, LMN2 antibody, LMNB antibody, MGC111419 antibody, LMNB1 antibody, lamin-B1 antibody, lamin B1 antibody

Cellular Localization

Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side

Background

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103292_39967_20170601_WB_KO_watermark_19011506_200.jpg

GTX103292 WB Image

KO/KD

Wild-type (WT) and Lamin B1 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody (GTX103292) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX103292_39967_IHC_19011506_179.jpg

GTX103292 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Lamin B1 antibody(GTX103292) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103292_39967_20150522_IP_19011506_174.jpg

GTX103292 IP Image

Immunoprecipitation of Lamin B1 protein from 293T whole cell extracts using 5 μg of Lamin B1 antibody (GTX103292).
Western blot analysis was performed using Lamin B1 antibody (GTX103292).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX103292_39967_WB_19011506_237.jpg

GTX103292 WB Image

Lamin B1 antibody detects Lamin B1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody (GTX103292) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Lamin B1 antibody Cat No. GTX103292

Rating ( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 2 users reviews)
Date : Anonymous submitted on 05-Jul-2017
Score :
Application Tested : WB
Sample Species : Hm
Sample : CHO-K1 cells
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Date : Anonymous submitted on 04-Jun-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 3*10^4 cells
Blocking : 4% Block Ace, 26°C, 1Hr
Primary Antibody : 1:500, 26°C, 1Hr
Lane Description : Lane 1: Huh7 nuclear extract.Lane 2: Huh7 cytoplasmic extract.
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169