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Lamin B1 antibody

KO/KD
Anti-Lamin B1 antibody used in Western Blot (WB). GTX103292
Anti-Lamin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103292
Anti-Lamin B1 antibody used in Immunoprecipitation (IP). GTX103292
Anti-Lamin B1 antibody used in Western Blot (WB). GTX103292

Cat No. GTX103292

Reference 25
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Hamster
Package
100 μl ($329),
25 μl ($169)
See all Lamin B1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , *Huh7

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lamin B1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lamin B1 , ADLD , LMN , LMN2 , LMNB

Cellular Localization

Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side

Background

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Lamin B1 antibody used in Western Blot (WB). GTX103292

GTX103292 WB Image

KO/KD

Wild-type (WT) and Lamin B1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody (GTX103292) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Lamin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103292

GTX103292 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Lamin B1 antibody(GTX103292) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Lamin B1 antibody used in Immunoprecipitation (IP). GTX103292

GTX103292 IP Image

Immunoprecipitation of Lamin B1 protein from 293T whole cell extracts using 5 μg of Lamin B1 antibody (GTX103292).
Western blot analysis was performed using Lamin B1 antibody (GTX103292).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Lamin B1 antibody used in Western Blot (WB). GTX103292

GTX103292 WB Image

Lamin B1 antibody detects Lamin B1 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody (GTX103292) diluted by 1:1000.

REFERENCE

Application Reference

Siew JJ et al. Nat Commun 2019; 10 (1) : 3473 Galectin-3 is required for the microglia-mediated brain inflammation in a model of Huntington's disease.
Application : WB
Reactivity : Mouse
Chen YR et al. Food Chem Toxicol 2019; 132 : 110693 Thyroid hormone-induced expression of inflammatory cytokines interfere with resveratrol-induced anti-proliferation of oral cancer cells.
Reactivity : Human
Silva B et al. Nat Commun 2019; 10 (1) : 2253 FANCM limits ALT activity by restricting telomeric replication stress induced by deregulated BLM and R-loops.
Application : WB
Reactivity : Human
Nana AW et al. Horm Cancer 2018; (Epub) Nano-Diamino-Tetrac (NDAT) Enhances Resveratrol-Induced Antiproliferation by Action on the RRM2 Pathway in Colorectal Cancers.
Application : WB
Reactivity : Human
Lin SJ et al. Food Chem Toxicol 2018; 120 : 43111 Nano-diamino-tetrac (NDAT) inhibits PD-L1 expression which is essential for proliferation in oral cancer cells.
Application : WB
Reactivity : Human
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Submit a Reference

REVIEW

Lamin B1 antibody Cat No. GTX103292

Rating
( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 4.5 based on 2 users reviews)
Anti-Lamin B1 antibody used in Western Blot (WB). GTX103292
Date : Anonymous submitted on 05-Jul-2017
Score :
Application Tested : WB
Sample Species : Hm
Sample : CHO-K1 cells
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-Lamin B1 antibody used in Western Blot (WB). GTX103292
Date : Anonymous submitted on 04-Jun-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 3*10^4 cells
Blocking : 4% Block Ace, 26°C, 1Hr
Primary Antibody : 1:500, 26°C, 1Hr
Lane Description : Lane 1: Huh7 nuclear extract.Lane 2: Huh7 cytoplasmic extract.
Submit a Review
Application Reference
Siew JJ et al. Nat Commun 2019; 10 (1):3473 Galectin-3 is required for the microglia-mediated brain inflammation in a model of Huntington's disease.
Application : WB
Reactivity : Mouse
Chen YR et al. Food Chem Toxicol 2019; 132:110693 Thyroid hormone-induced expression of inflammatory cytokines interfere with resveratrol-induced anti-proliferation of oral cancer cells.
Reactivity : Human
Silva B et al. Nat Commun 2019; 10 (1):2253 FANCM limits ALT activity by restricting telomeric replication stress induced by deregulated BLM and R-loops.
Application : WB
Reactivity : Human
Nana AW et al. Horm Cancer 2018; (Epub) Nano-Diamino-Tetrac (NDAT) Enhances Resveratrol-Induced Antiproliferation by Action on the RRM2 Pathway in Colorectal Cancers.
Application : WB
Reactivity : Human
Lin SJ et al. Food Chem Toxicol 2018; 120:43111 Nano-diamino-tetrac (NDAT) inhibits PD-L1 expression which is essential for proliferation in oral cancer cells.
Application : WB
Reactivity : Human
Chou HC et al. Life Sci 2018; (Epub) Proteomic analysis of honokiol-induced cytotoxicity in thyroid cancer cells.
Application : WB
Reactivity : Human
Chin YT et al. Endocr Relat Cancer 2018; 25 (5):533-545 Thyroxine inhibits resveratrol-caused apoptosis by PD-L1 in ovarian cancer cells.
Application : WB
Reactivity : Human
Conde J et al. Cell Physiol Biochem 2018; 45 (6):2401-2410 E74-Like Factor (ELF3) and Leptin, a Novel Loop Between Obesity and Inflammation Perpetuating a Pro-Catabolic State in Cartilage.
Application : WB
Reactivity : Human
Chen Q et al. J Nat Med 2018; (Epub) Scutellaria baicalensis regulates FFA metabolism to ameliorate NAFLD through the AMPK-mediated SREBP signaling pathway.
Application : WB
Reactivity : Human
Lee YS et al. Horm Cancer 2018; (Epub) Thyroid Hormone Promotes β-Catenin Activation and Cell Proliferation in Colorectal Cancer.
Application : WB
Reactivity : Human
Hsieh MT et al. Oncotarget 2017; 8 (15):24237-24249 Crosstalk between integrin αvβ3 and ERα contributes to thyroid hormone-induced proliferation of ovarian cancer cells.
Application : WB
Reactivity : Human
Cheng TM et al. Food Chem Toxicol 2017; (Epub) Resveratrol induces sumoylated COX-2-dependent anti-proliferation in human prostate cancer LNCaP cells.
Application : WB
Reactivity : Human
Hsieh CL et al. Sci Rep 2017; 7 (1):9065 Reactive oxygen species-mediated switching expression of MMP-3 in stromal fibroblasts and cancer cells during prostate cancer progression.
Application : WB
Reactivity : Human
Nickel S et al. Pharm Res 2017; (Epub) Expression and Activity of Breast Cancer Resistance Protein (BCRP/ABCG2) in Human Distal Lung Epithelial Cells In Vitro.
Application : WB
Reactivity : Human
Tsai TH et al. Molecules 2017; 22 (5) Protective Effect of Caffeic Acid Derivatives on tert-Butyl Hydroperoxide-Induced Oxidative Hepato-Toxicity and Mitochondrial Dysfunction in HepG2 Cells.
Application : WB
Reactivity : Human
Chao W et al. Molecules 2017; 22 (4) 3,4-Dihydroxybenzalactone Suppresses Human Non-Small Cell Lung Carcinoma Cells Metastasis via Suppression of Epithelial to Mesenchymal Transition, ROS-Mediated PI3K/AKT/MAPK/MMP and NFκB Signaling Pathways.
Application : WB
Reactivity : Human
Zanini IM et al. J Cell Sci 2017; (Epub) Human Cactin interacts with DHX8 and SRRM2 to assure efficient pre-mRNA splicing and sister chromatid cohesion.
Application : WB
Reactivity : Human
Choi JH et al. Oncogenesis 2016; 5:e221 Characterization of MUDENG, a novel anti-apoptotic protein.
Application : WB
Reactivity : Human
Santoro A et al. PLoS One 2015; 10 (8):e0135979 SERPINE2 Inhibits IL-1α-Induced MMP-13 Expression in Human Chondrocytes: Involvement of ERK/NF-κB/AP-1 Pathways.
Application : WB
Reactivity : Human
Mandal SM et al. Mol Biosyst 2015; 11 (9):238018 * and * directly interact with dna and induce oxidative damage in the mammalian genome.
Application : WB
Reactivity : Human
Lee JC et al. Br J Pharmacol 2014; 171 (1):237-52 Andrographolide exerts anti-hepatitis C virus activity by up-regulating haeme oxygenase-1 via the p38 MAPK/Nrf2 pathway in human hepatoma cells.
Application : WB
Reactivity : Human
Sobolesky PM et al. PLoS One 2014; 9 (9):e107530 Regulation of the tumor suppressor FOXO3 by the thromboxane-A2 receptors in urothelial cancer.
Arora R et al. Nat Commun 2014; 5:5220 RNaseH1 regulates TERRA-telomeric DNA hybrids and telomere maintenance in ALT tumour cells.
Application : WB
Reactivity : Human
Chang YL et al. Endocr Relat Cancer 2014; Regulation of estrogen receptor α function in oral squamous cell carcinoma cells by FAK signaling.
Li HC et al. PLoS Pathog 2010; 6 (10):e1001162 Nuclear export and import of human hepatitis B virus capsid protein and particles.
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169