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Lamin B1 antibody

Orthogonal
Anti-Lamin B1 antibody used in Western Blot (WB). GTX54288
Anti-Lamin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54288
Anti-Lamin B1 antibody used in Western Blot (WB). GTX54288
Anti-Lamin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54288
Anti-Lamin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54288
Anti-Lamin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54288
Anti-Lamin B1 antibody used in ChIP assay (ChIP assay). GTX54288
Anti-Lamin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54288
Anti-Lamin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54288
Anti-Lamin B1 antibody used in Immunoprecipitation (IP). GTX54288

Cat. No. GTX54288

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Lamin B1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 397-586 of human LMNB1 (NP_005564.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lamin B1 , ADLD , LMN , LMN2 , LMNB

Background

This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-Lamin B1 antibody used in Western Blot (WB). GTX54288

GTX54288 WB Image

Orthogonal

WB analysis of various cell lysates using GTX54288 Lamin B1 antibody. Jurkat cells were treated by Etoposide (25 μM) at 37ºC for 5 hours.
Loading: 25μg per lane
Dilution : 1:1000

Anti-Lamin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54288

GTX54288 ICC/IF Image

ICC/IF analysis of PC-12 cells using GTX54288 Lamin B1 antibody.
Dilution : 1:150
Blue : DAPI

Anti-Lamin B1 antibody used in Western Blot (WB). GTX54288

GTX54288 WB Image

Orthogonal

WB analysis of various cell lysates using GTX54288 Lamin B1 antibody. L929 cells were treated by staurosporine(1 μM) for 3 hour.
Loading: 25μg per lane
Dilution : 1:1000

Anti-Lamin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54288

GTX54288 IHC-P Image

IHC-P analysis of rat brain tissue using GTX54288 Lamin B1 antibody.
Antigen retrieval : 10 mM citrate buffer (pH 6.0)
Dilution : 1:250

Anti-Lamin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54288

GTX54288 IHC-P Image

IHC-P analysis of human esophageal cancer tissue using GTX54288 Lamin B1 antibody.
Antigen retrieval : 10 mM citrate buffer (pH 6.0)
Dilution : 1:250

Anti-Lamin B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54288

GTX54288 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX54288 Lamin B1 antibody.
Antigen retrieval : 10 mM citrate buffer (pH 6.0)
Dilution : 1:250

Anti-Lamin B1 antibody used in ChIP assay (ChIP assay). GTX54288

GTX54288 ChIP assay Image

ChIP analysis of HeLa cell using GTX54288 Lamin B1 antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR.

Anti-Lamin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54288

GTX54288 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54288 Lamin B1 antibody.
Dilution : 1:150
Blue : DAPI

Anti-Lamin B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54288

GTX54288 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX54288 Lamin B1 antibody.
Dilution : 1:200
Blue : DAPI

Anti-Lamin B1 antibody used in Immunoprecipitation (IP). GTX54288

GTX54288 IP Image

IP analysis of HeLa cell lysates using GTX54288 Lamin B1 antibody.
Total extraction : 300 μg
IP antibody amount : 3μg
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399