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Lamin B2 antibody [GT144]

KO/KD Orthogonal
Anti-Lamin B2 antibody [GT144] used in Western Blot (WB). GTX628803
Anti-Lamin B2 antibody [GT144] used in Immunoprecipitation (IP). GTX628803
Anti-Lamin B2 antibody [GT144] used in Western Blot (WB). GTX628803
Anti-Lamin B2 antibody [GT144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628803
Anti-Lamin B2 antibody [GT144] used in IHC (Paraffin sections) (IHC-P). GTX628803
Anti-Lamin B2 antibody [GT144] used in Western Blot (WB). GTX628803

Cat. No. GTX628803

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT144

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Lamin B2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , HepG2 , HepG2 nuclear

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lamin B2. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888055

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lamin B2 , EPM9 , LAMB2 , LMN2

Cellular Localization

Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side

Background

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B2. This gene is in a head-to-tail orientation with the gene for the translocase of inner mitochondrial membrane 13 homolog gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Lamin B2 antibody [GT144] used in Western Blot (WB). GTX628803

GTX628803 WB Image

KO/KD

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B2 antibody [GT144] (GTX628803) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Lamin B2 antibody [GT144] used in Immunoprecipitation (IP). GTX628803

GTX628803 IP Image

Lamin B2 antibody [GT144] immunoprecipitates Lamin B2 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of Lamin B2 protein expressing HepG2 cells B : Control with 3 μg of pre-immune mouse IgG C : Immunoprecipitation of Lamin B2 by 3 μg of Lamin B2 antibody [GT144] (GTX628803) 7.5% SDS-PAGE The immunoprecipitated Lamin B2 protein was detected by Lamin B2 antibody [GT144] (GTX628803) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221667-01) was used as a secondary reagent.

Anti-Lamin B2 antibody [GT144] used in Western Blot (WB). GTX628803

GTX628803 WB Image

Lamin B2 antibody [GT144] detects LMNB2 protein by western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
B. 30 μg SK-N-SH whole cell lysate/extract
C. 30 μg IMR32 whole cell lysate/extract
D. 30 μg SK-N-AS whole cell lysate/extract
7.5% SDS-PAGE
Lamin B2 antibody [GT144] (GTX628803) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Lamin B2 antibody [GT144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628803

GTX628803 ICC/IF Image

Lamin B2 antibody [GT144] detects LMNB2 protein at nuclear envelope by confocal immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde for 30 min.
Red: LMNB2 protein stained by Lamin B2 antibody [GT144] (GTX628803) diluted at 1:1000.
Green: alpha Tubulin antibody (GTX102078) diluted at 1:1000.
Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope]

Anti-Lamin B2 antibody [GT144] used in IHC (Paraffin sections) (IHC-P). GTX628803

GTX628803 IHC-P Image

Lamin B2 antibody [GT144] detects LMNB2 protein at nucleus membrane on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
Lamin B2 antibody [GT144] (GTX628803) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Lamin B2 antibody [GT144] used in Western Blot (WB). GTX628803

GTX628803 WB Image

Orthogonal

Lamin B2 antibody [GT144] detects LMNB2 protein by western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract
B. 30 μg HepG2 cytosol lysate/extract
C. 30 μg HepG2 nuclear lysate/extract
7.5% SDS-PAGE
Lamin B2 antibody [GT144] (GTX628803) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Jevti? P et al. EMBO Rep 2019; 20 (6) The nucleoporin ELYS regulates nuclear size by controlling NPC number and nuclear import capacity.
Yang SL et al. Oncotarget 2017; 8 (62) : 105860-105872 Andrographolide suppresses the migratory ability of human glioblastoma multiforme cells by targeting ERK1/2-mediated matrix metalloproteinase-2 expression.
Application : WB
Reactivity : Human
Urban V et al. J Cell Biol 2016; 214 (4) : 401-15 RECQ5 helicase promotes resolution of conflicts between replication and transcription in human cells.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations