APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
U87-MG , SK-N-SH , IMR32 , SK-N-AS , HepG2 , HepG2 nuclear
Predict Reactivity
Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.81 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Lamin B2. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888055
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
lamin B2 , EPM9 , LAMB2 , LMN2
Cellular Localization
Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side
Background
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B2. This gene is in a head-to-tail orientation with the gene for the translocase of inner mitochondrial membrane 13 homolog gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX628803 WB Image
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B2 antibody [GT144] (GTX628803) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
|
GTX628803 IP Image
Lamin B2 antibody [GT144] immunoprecipitates Lamin B2 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of Lamin B2 protein expressing HepG2 cells B : Control with 3 μg of pre-immune mouse IgG C : Immunoprecipitation of Lamin B2 by 3 μg of Lamin B2 antibody [GT144] (GTX628803) 7.5% SDS-PAGE The immunoprecipitated Lamin B2 protein was detected by Lamin B2 antibody [GT144] (GTX628803) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221667-01) was used as a secondary reagent.
|
|
GTX628803 WB Image
Lamin B2 antibody [GT144] detects LMNB2 protein by western blot analysis. A. 30 μg U87-MG whole cell lysate/extract B. 30 μg SK-N-SH whole cell lysate/extract C. 30 μg IMR32 whole cell lysate/extract D. 30 μg SK-N-AS whole cell lysate/extract 7.5% SDS-PAGE Lamin B2 antibody [GT144] (GTX628803) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
|
GTX628803 ICC/IF Image
Lamin B2 antibody [GT144] detects LMNB2 protein at nuclear envelope by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde for 30 min. Red: LMNB2 protein stained by Lamin B2 antibody [GT144] (GTX628803) diluted at 1:1000. Green: alpha Tubulin antibody (GTX102078) diluted at 1:1000. Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope]
|
|
GTX628803 IHC-P Image
Lamin B2 antibody [GT144] detects LMNB2 protein at nucleus membrane on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. Lamin B2 antibody [GT144] (GTX628803) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
GTX628803 WB Image
Lamin B2 antibody [GT144] detects LMNB2 protein by western blot analysis. A. 30 μg HepG2 whole cell lysate/extract B. 30 μg HepG2 cytosol lysate/extract C. 30 μg HepG2 nuclear lysate/extract 7.5% SDS-PAGE Lamin B2 antibody [GT144] (GTX628803) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
REFERENCE
REVIEW
There are currently no reviews for Lamin B2 antibody [GT144] (GTX628803). Be the first to share your experience with this product.