Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B2 antibody [N3C2], Internal (GTX109894) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Lamin B2 antibody [N3C2], Internal immunoprecipitates Lamin B2 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of Lamin B2 protein expressing HepG2 cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of Lamin B2 by 3 μg of Lamin B2 antibody [N3C2], Internal (GTX109894) 7.5% SDS-PAGE The immunoprecipitated Lamin B2 protein was detected by Lamin B2 antibody [N3C2], Internal (GTX109894) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Lamin B2 antibody [N3C2], Internal detects LMNB2 protein at on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
Lamin B2 antibody [N3C2], Internal (GTX109894) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Lamin B2 antibody [N3C2], Internal detects LMNB2 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
7.5% SDS-PAGE
Lamin B2 antibody [N3C2], Internal (GTX109894) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.