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Lamin B2 antibody [N3C2], Internal

Anti-Lamin B2 antibody [N3C2], Internal used in Western Blot (WB). GTX109894
Anti-Lamin B2 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX109894
Anti-Lamin B2 antibody [N3C2], Internal used in Immunoprecipitation (IP). GTX109894
Anti-Lamin B2 antibody [N3C2], Internal used in Western Blot (WB). GTX109894

Cat. No. GTX109894

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Lamin B2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:40000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lamin B2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950821

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lamin B2 , EPM9 , LAMB2 , LMN2

Cellular Localization

Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side

Background

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B2. This gene is in a head-to-tail orientation with the gene for the translocase of inner mitochondrial membrane 13 homolog gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Lamin B2 antibody [N3C2], Internal used in Western Blot (WB). GTX109894

GTX109894 WB Image

Lamin B2 antibody [N3C2], Internal detects LMNB2 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
7.5% SDS-PAGE
Lamin B2 antibody [N3C2], Internal (GTX109894) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Lamin B2 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX109894

GTX109894 IHC-P Image

Lamin B2 antibody [N3C2], Internal detects LMNB2 protein at on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
Lamin B2 antibody [N3C2], Internal (GTX109894) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Lamin B2 antibody [N3C2], Internal used in Immunoprecipitation (IP). GTX109894

GTX109894 IP Image

Lamin B2 antibody [N3C2], Internal immunoprecipitates Lamin B2 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of Lamin B2 protein expressing HepG2 cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of Lamin B2 by 3 μg of Lamin B2 antibody [N3C2], Internal (GTX109894) 7.5% SDS-PAGE The immunoprecipitated Lamin B2 protein was detected by Lamin B2 antibody [N3C2], Internal (GTX109894) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-Lamin B2 antibody [N3C2], Internal used in Western Blot (WB). GTX109894

GTX109894 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B2 antibody [N3C2], Internal (GTX109894) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Shih JW et al. Nat Commun 2017; 8 : 15874 Long noncoding RNA LncHIFCAR/MIR31HG is a HIF-1α co-activator driving oral cancer progression.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169