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Lamin B2 antibody

Anti-Lamin B2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110309
Anti-Lamin B2 antibody used in Western Blot (WB). GTX110309
Anti-Lamin B2 antibody used in IHC (Paraffin sections) (IHC-P). GTX110309

Cat No. GTX110309

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lamin B2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10720644

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lamin B2 , EPM9 , LAMB2 , LMN2

Cellular Localization

Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side

Background

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B2. This gene is in a head-to-tail orientation with the gene for the translocase of inner mitochondrial membrane 13 homolog gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Lamin B2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110309

GTX110309 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Lamin B2(GTX110309) antibody (Green) at 1:500 dilution.

Anti-Lamin B2 antibody used in Western Blot (WB). GTX110309

GTX110309 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX110309 diluted at 1:10000

Anti-Lamin B2 antibody used in IHC (Paraffin sections) (IHC-P). GTX110309

GTX110309 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using Lamin B2(GTX110309) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169