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Laminin alpha 2 antibody

Anti-Laminin alpha 2 antibody used in Western Blot (WB). GTX12391

Cat No. GTX12391

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse, Rat
Package
100 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.5μg/ml
Not tested in other applications.

Calculated MW

343.9 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

2.5% BSA, 0.45% NaCl, 0.1% Na₂HPO₄, 0.025% Thimerosal, 0.025% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

500 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Laminin 2 alpha(152-170aa WILERSLDDVEYKPWQYHA), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Secreted,Extracellular space,Extracellular matrix,Basement membrane

Background

Laminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Laminin alpha 2 antibody used in Western Blot (WB). GTX12391

GTX12391 WB Image

WB analysis of various samples using GTX12391 Laminin alpha 2 antibody.
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : PANC cell lysate

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Thimerosal.pdf
Package List Price ($)
$ 319