Laminin antibody

Cat. No. GTX27463

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, IHC (Free Floating)
Reactivity	Human, Mouse, Rat, Rabbit, Sheep, Insect
Package	100 μl ($649)

See all Laminin products

View product citations for antibody GTX27463 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:100 - 1:5000
ICC/IF	1:50 - 1:200
IHC-P	1:500 - 1:2000
IHC-Fr	1:500 - 1:2000
IHC (Free Floating)	Assay dependent

Not tested in other applications.

Product Note

This is pan-specific and reacts well with all Laminin isoforms tested: Laminin-1 (alpha-1, beta-1, and gamma-1) and Laminin-2 (alpha-2, beta-1, and gamma-1).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Laminin isolated from mouse EHS tumor. [UniProt# P19137]

Purification

Purified IgG

Conjugation

Unconjugated

RRID

AB_384151

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Laminin antibodies are widely used to label blood vessels and basement membranes.

Research Area

DATA IMAGES

GTX27463 IHC-P Image

IHC-P analysis of rat spinal cord and dorsal root tissue using GTX27463 Laminin Antibody.
Antigen retrieval : Pepsin antigen retrieval

GTX27463 WB Image

WB analysis of rat heart cell lysates (lane 1) and purified laminin protein from mouse EHS sarcoma (lane 2) using GTX27463 Laminin Antibody. This antibody recognizes 3 laminin isotypes: alpha 1 (440kDa), beta 1 (220kD) and gamma 1 (220kDa). Also recognized is a laminin binding protein at 120kDa in both rat heart lysates and purified laminin protein. Since this protein always coexpresses with laminin this crossreactivity is irrelevant.

GTX27463 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX27463 Laminin Antibody.
Green : primary antibody
Red : Actin
Blue : DAPI
Dilution : 1:5

GTX27463 IHC (Free Floating) Image

IHC-FrFl analysis of mouse cortex tissue section using GTX27463 Laminin Antibody. This antibody reveals strong staining in the basement membranes of blood vessels.