GeneTex
  • Country / Location Selection

United States (US)

Laminin beta 2 antibody, C-term

Anti-Laminin beta 2 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81697
Anti-Laminin beta 2 antibody, C-term used in Western Blot (WB). GTX81697
Anti-Laminin beta 2 antibody, C-term used in Flow cytometry (FACS). GTX81697

Cat. No. GTX81697

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, FACS

Reactivity

Human
Package
400 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:50-1:100
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

196 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 1579-1606 amino acids from the C-terminal region of human LAMB2.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

laminin subunit beta 2 , LAMS , NPHS5

Background

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 2. The beta 2 chain contains the 7 structural domains typical of beta chains of laminin, including the short alpha region. However, unlike beta 1 chain, beta 2 has a more restricted tissue distribution. It is enriched in the basement membrane of muscles at the neuromuscular junctions, kidney glomerulus and vascular smooth muscle. Transgenic mice in which the beta 2 chain gene was inactivated by homologous recombination, showed defects in the maturation of neuromuscular junctions and impairment of glomerular filtration. Alternative splicing involving a non consensus 5' splice site (gc) in the 5' UTR of this gene has been reported. It was suggested that inefficient splicing of this first intron, which does not change the protein sequence, results in a greater abundance of the unspliced form of the transcript than the spliced form. The full-length nature of the spliced transcript is not known. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-Laminin beta 2 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81697

GTX81697 IHC-P Image

IHC-P analysis of human hepatocarcinoma using GTX81697 Laminin beta 2 antibody, C-term.

Anti-Laminin beta 2 antibody, C-term used in Western Blot (WB). GTX81697

GTX81697 WB Image

WB analysis of various samples using GTX81697 Laminin beta 2 antibody, C-term.
Lane 1: HepG2 whole cell lysate
Lane 2: Jurkat whole cell lysate
Loading : 20 μg per lane
Dilution : 1:1000

Anti-Laminin beta 2 antibody, C-term used in Flow cytometry (FACS). GTX81697

GTX81697 FACS Image

FACS analysis of HepG2 cells using GTX81697 Laminin beta 2 antibody, C-term.
Top histogram : negative control
Bottom histogram : HepG2 cells

REFERENCE

There are currently no references for Laminin beta 2 antibody, C-term (GTX81697). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Laminin beta 2 antibody, C-term (GTX81697). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399