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Laminin beta 3 antibody [C1C2], Internal

GTX103736_43118_20180216_WB_19011506_154.jpg
GTX103736_43118_20180314_ICC_IF_19011506_805.jpg
GTX103736_39904_IHC_19011506_581.jpg
GTX103736_WB_M_19011506_589.jpg

Cat No. GTX103736

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

130 kDa. ( Note )

Positive Control

A431 , NIH-3T3 , *mouse bone marrow

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Laminin beta 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Laminin Subunit Beta 3,Ai1A,Bm600-125Kda,Lam5,Lamnb1,Lamb3

Cellular Localization

Secreted , extracellular space , extracellular matrix , basement membrane

Background

The product encoded by this gene is a laminin that belongs to a family of basement membrane proteins. This protein is a beta subunit laminin, which together with an alpha and a gamma subunit, forms laminin-5. Mutations in this gene cause epidermolysis bullosa junctional Herlitz type, and generalized atrophic benign epidermolysis bullosa, diseases that are characterized by blistering of the skin. Multiple alternatively spliced transcript variants that encode the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103736_43118_20180216_WB_19011506_154.jpg

GTX103736 WB Image

Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with Laminin beta 3 antibody [C1C2], Internal (GTX103736) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103736_43118_20180314_ICC_IF_19011506_805.jpg

GTX103736 ICC/IF Image

Laminin beta 3 antibody [C1C2], Internal detects Laminin beta 3 protein by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Laminin beta 3 stained by Laminin beta 3 antibody [C1C2], Internal (GTX103736) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX103736_39904_IHC_19011506_581.jpg

GTX103736 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung cancer, using Laminin beta 3(GTX103736) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103736_WB_M_19011506_589.jpg

GTX103736 WB Image

Sample (30 µg of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX103736 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Laminin beta 3 antibody [C1C2], Internal Cat No. GTX103736

Rating ( Average 4 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 29-Apr-2015
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : bone marrow cells
Blocking : PBS with 1% triton and 10% BSA, 25°C, 1Hr
Fixation : 10% Formaline
Permeabilization /Antigen retrieval : TBST
Primary Antibody : 1:200, 4°C, 18Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169