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Lck antibody [C1C3]

Anti-Lck antibody [C1C3] used in Western Blot (WB). GTX101275
Anti-Lck antibody [C1C3] used in Western Blot (WB). GTX101275

Cat No. GTX101275

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all Lck products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

H1299 , NIH-3T3

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Lck. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

LCK proto-oncogene, Src family tyrosine kinase , IMD22 , LSK , YT16 , p56lck , pp58lck

Cellular Localization

Cytoplasm , Cell membrane; Lipid-anchor; Cytoplasmic side

Background

This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Lck antibody [C1C3] used in Western Blot (WB). GTX101275

GTX101275 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
7.5% SDS PAGE

GTX101275 diluted at 1:1000

Anti-Lck antibody [C1C3] used in Western Blot (WB). GTX101275

GTX101275 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Lck antibody [C1C3] (GTX101275) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169