APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
H1299 , NIH-3T3
Predict Reactivity
Rat, Zebrafish, Sheep, Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Lck. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Lck Proto-Oncogene, Src Family Tyrosine Kinase,Imd22,Lsk,Yt16,P56Lck,Pp58Lck,Lck
Cellular Localization
Cytoplasm , Cell membrane; Lipid-anchor; Cytoplasmic side
Background
This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX101275 WB Image
Sample(30 μg of whole cell lysate)
A:H1299
7.5% SDS PAGE
GTX101275 diluted at 1:500
|
|
GTX101275 WB Image
Sample (30 ug of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX101275 diluted at 1:1000
|
