APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse, Rat, Zebrafish, Sheep, Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.61 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Lck. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950803
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
LCK proto-oncogene, Src family tyrosine kinase , IMD22 , LSK , YT16 , p56lck , pp58lck
Cellular Localization
Cytoplasm , Cell membrane; Lipid-anchor; Cytoplasmic side
Background
This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Lck antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101275](/templates/default/js/lightbox/images/loading.gif "Anti-Lck antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101275")
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GTX101275 ICC/IF Image
Lck antibody [C1C3] detects Lck protein at cell membrane by immunofluorescent analysis.Sample: MOLT-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Lck stained by Lck antibody [C1C3] (GTX101275) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
There are currently no references for Lck antibody [C1C3] (GTX101275). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Lck antibody [C1C3] (GTX101275). Be the first to share your experience with this product.
![Anti-Lck antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101275](/upload/website/prouct_img/small/GTX101275/GTX101275_43628_20230512_ICC_IF_23060622_378.webp "Anti-Lck antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101275")
![Anti-Lck antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101275](/upload/website/prouct_img/normal/GTX101275/GTX101275_43628_20230512_ICC_IF_23060622_378.webp "Anti-Lck antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101275")