GeneTex
United States (US)

Lck antibody [LCK-01]

Lck-antibody-LCK-01__GTX28075-1_18121411_466.jpg

Cat No. GTX28075

Host Mouse
Clonality Monoclonal
Clone Name LCK-01
Isotype IgG1
Application WB, ICC/IF, FACS, IP
Reactivity Human
APPLICATION

Application Note

FACS: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

58 kDa. ( Note )

Positive Control

Jurkat cell lysate

Specificity/Sensitivity

The antibody recognizes defined epitope (aa 22-36) on p56lck.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, corresponding to amino acids 22-36 of Human Lck p56.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

LSK, YT16, p56lck, pp58lck

Cellular Localization

Cytoplasmic

Background

Lck (p56lck), a member of the Src family of non-receptor tyrosine protein kinases, is expressed predominantly in T cells. Lck function is critical both for T cell development in the thymus and activation of mature T cells in the periphery by antigen. The activity of Lck is known to be regulated by phosphorylation of two conserved tyrosine residues, Tyr-505 (equivalent to Tyr-529 in c-Src) and Tyr-394 (equivalent to Tyr-418 in c-Src).

Database

Research Area

DATA IMAGES
Lck-antibody-LCK-01__GTX28075-1_18121411_466.jpg

GTX28075 WB Image

Western Blot analysis (reducing conditions) of human Lck in whole cell lysate using anti-human Lck (LCK-01) (GTX28075).
Lane 1: J. CaM-1.6 cell line (a mutant derivate of the JURKAT cell line) transfected with Lck
Lane 2: HEK-293T cell line transfected with Lck
Lane 3: HEK-293T cell line (non-transfected)

Package List Price ($)
$ 319