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Lck antibody [N3C3]

IP/MS
Anti-Lck antibody [N3C3] used in Western Blot (WB). GTX107785
Anti-Lck antibody [N3C3] used in Western Blot (WB). GTX107785

Cat. No. GTX107785

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse
Package
100 μl ($419),
25 μl ($169)
See all Lck products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP Assay dependent
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

293T , Molt-4

Product Note

IP/MS validation was supported by references (PMID:30377389)

Predict Reactivity

Rat, Sheep, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lck. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950804

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

LCK proto-oncogene, Src family tyrosine kinase , IMD22 , LSK , YT16 , p56lck , pp58lck

Cellular Localization

Cytoplasm , Cell membrane; Lipid-anchor; Cytoplasmic side

Background

This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Lck antibody [N3C3] used in Western Blot (WB). GTX107785

GTX107785 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Lck antibody [N3C3] (GTX107785) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Lck antibody [N3C3] used in Western Blot (WB). GTX107785

GTX107785 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX107785 diluted at 1:1000

REFERENCE

Application Reference

Hu XX et al. Acta Pharmacol Sin 2022; 43 (2) : 387-400 An IgD-Fc-Ig fusion protein restrains the activation of T and B cells by inhibiting IgD-IgDR-Lck signaling in rheumatoid arthritis.
Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169