GeneTex
United States (US)

Lck antibody [N3C3]

GTX107785_WB_19011507_657.jpg

Cat No. GTX107785

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP Assay dependent
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377389)

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lck. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Lck Proto-Oncogene, Src Family Tyrosine Kinase,Imd22,Lsk,Yt16,P56Lck,Pp58Lck,Lck

Cellular Localization

Cytoplasm , Cell membrane; Lipid-anchor; Cytoplasmic side

Background

This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX107785_WB_19011507_657.jpg

GTX107785 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX107785 diluted at 1:1000

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169