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Lck (phospho Tyr394) antibody

Orthogonal
Anti-Lck (phospho Tyr394) antibody used in Western Blot (WB). GTX133876
Anti-Lck (phospho Tyr394) antibody used in Western Blot (WB). GTX133876
Anti-Lck (phospho Tyr394) antibody used in Western Blot (WB). GTX133876

Cat. No. GTX133876

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Lck products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

Jurkat , PC-12 , Rat2

Predict Reactivity

Zebrafish, Sheep, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.09 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr394 of human Lck. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887129

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

LCK proto-oncogene, Src family tyrosine kinase , IMD22 , LSK , YT16 , p56lck , pp58lck

Cellular Localization

Cytoplasm , Cell membrane; Lipid-anchor; Cytoplasmic side

Background

This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Lck (phospho Tyr394) antibody used in Western Blot (WB). GTX133876

GTX133876 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Lck (phospho Tyr394) antibody (GTX133876) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Lck (phospho Tyr394) antibody used in Western Blot (WB). GTX133876

GTX133876 WB Image

Orthogonal

Untreated (–) and treated (+) Jurkat whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lck (phospho Tyr394) antibody (GTX133876) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Lck (phospho Tyr394) antibody used in Western Blot (WB). GTX133876

GTX133876 WB Image

The data was published in the journal Nat Commun in 2017. PMID: 28970470

REFERENCE

Application Reference

Hu XX et al. Acta Pharmacol Sin 2022; 43 (2) : 387-400 An IgD-Fc-Ig fusion protein restrains the activation of T and B cells by inhibiting IgD-IgDR-Lck signaling in rheumatoid arthritis.
He QW et al. J Nat Prod 2020; 83 (9) : 2578-2586 Synthesis and Biological Evaluation of Celastrol Derivatives as Potential Immunosuppressive Agents.
Application : WB
Reactivity : Mouse
Duan Y et al. Cell Mol Immunol 2020; CFTR is a negative regulator of γδ T cell IFN-γ production and antitumor immunity.
Application : WB
Reactivity : Mouse
Marklin M et al. Nat Commun 2017; 8 (1) : 755 NFAT2 is a critical regulator of the anergic phenotype in chronic lymphocytic leukaemia.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169