Boiled and unboiled HepG2 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Leptin Receptor antibody [GT1233] (GTX02830) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

IHC-P analysis of human liver cancer tissue section using GTX02830 Leptin Receptor antibody [GT1233].
Antigen retrieval : high pressure antigen retrieval with 10 mM citrate buffer pH 6.0
Dilution : 1:200

IHC-P analysis of human kidney tissue section using GTX02830 Leptin Receptor antibody [GT1233].
Antigen retrieval : high pressure antigen retrieval with 10 mM citrate buffer pH 6.0
Dilution : 1:200

IHC-P analysis of human tonsil tissue section using GTX02830 Leptin Receptor antibody [GT1233].
Antigen retrieval: high pressure antigen retrieval with 10 mM citrate buffer pH 6.0
Dilution : 1:200

WB analysis of various samples using GTX02830 Leptin Receptor antibody [GT1233].
Dilution : 1:1000
Loading : 25μg