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Leptin Receptor antibody [GT1233]

Recombinant antibody Orthogonal
Anti-Leptin Receptor antibody [GT1233] used in Western Blot (WB). GTX02830
Anti-Leptin Receptor antibody [GT1233] used in Western Blot (WB). GTX02830
Anti-Leptin Receptor antibody [GT1233] used in IHC (Paraffin sections) (IHC-P). GTX02830
Anti-Leptin Receptor antibody [GT1233] used in IHC (Paraffin sections) (IHC-P). GTX02830
Anti-Leptin Receptor antibody [GT1233] used in IHC (Paraffin sections) (IHC-P). GTX02830

Cat. No. GTX02830

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1233

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419)
See all Leptin Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

132 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Leptin Receptor

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

leptin receptor , CD295 , LEP-R , LEPRD , OB-R , OBR

Cellular Localization

Cell membrane

Background

The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010]

Database

Research Area

DATA IMAGES

Anti-Leptin Receptor antibody [GT1233] used in Western Blot (WB). GTX02830

GTX02830 WB Image

Orthogonal

Boiled and unboiled HepG2 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Leptin Receptor antibody [GT1233] (GTX02830) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Anti-Leptin Receptor antibody [GT1233] used in Western Blot (WB). GTX02830

GTX02830 WB Image

WB analysis of various samples using GTX02830 Leptin Receptor antibody [GT1233].
Dilution : 1:1000
Loading : 25μg

Anti-Leptin Receptor antibody [GT1233] used in IHC (Paraffin sections) (IHC-P). GTX02830

GTX02830 IHC-P Image

IHC-P analysis of human tonsil tissue section using GTX02830 Leptin Receptor antibody [GT1233].
Antigen retrieval: high pressure antigen retrieval with 10 mM citrate buffer pH 6.0
Dilution : 1:200

Anti-Leptin Receptor antibody [GT1233] used in IHC (Paraffin sections) (IHC-P). GTX02830

GTX02830 IHC-P Image

IHC-P analysis of human kidney tissue section using GTX02830 Leptin Receptor antibody [GT1233].
Antigen retrieval : high pressure antigen retrieval with 10 mM citrate buffer pH 6.0
Dilution : 1:200

Anti-Leptin Receptor antibody [GT1233] used in IHC (Paraffin sections) (IHC-P). GTX02830

GTX02830 IHC-P Image

IHC-P analysis of human liver cancer tissue section using GTX02830 Leptin Receptor antibody [GT1233].
Antigen retrieval : high pressure antigen retrieval with 10 mM citrate buffer pH 6.0
Dilution : 1:200

REFERENCE

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REVIEW

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Package List Price ($)
$ 419