APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-1μg/ml |
| 4μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89338-PEP).
Product Note
This antibody is expected to recognise all three reported isoforms (NP_002294.2; NP_001003679.1; NP_001003680.1).
Predict Reactivity
Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-TQDDIEKHQSDAG, from the internal region of the protein sequence according to NP_002294.2; NP_001003679.1; NP_001003680.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
leptin receptor , CD295 , LEP-R , LEPRD , OB-R , OBR
Cellular Localization
Cell membrane
Background
The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010]
Database
Research Area
DATA IMAGES
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GTX89338 IHC-P Image
IHC-P analysis of human heart using GTX89338 Leptin Receptor antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 4μg/ml
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GTX89338 WB Image
WB analysis of human cerebral cortex lysate using GTX89338 Leptin Receptor antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89338 WB Image
WB analysis of mouse (A) and rat (B) brain lysate using GTX89338 Leptin Receptor antibody, Internal.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89338 IHC-P Image
IHC-P analysis of human liver using GTX89338 Leptin Receptor antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 4μg/ml
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REFERENCE
There are currently no references for Leptin Receptor antibody, Internal (GTX89338). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Leptin Receptor antibody, Internal (GTX89338). Be the first to share your experience with this product.
