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Leptin Receptor antibody

Cat. No. GTX10979

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-Fr, FACS

Reactivity

Mouse
Package
50 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

131 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 5% Trehalose

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

recombinant mouse leptin receptor expressed in NS0 cells.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

leptin receptor , LEPROT , Leprb , Modb1 , OB-RGRP , Obr , db , diabetes , obese-like , obl

Cellular Localization

Cell membrane

Background

Leptin is the adipocyte-specific product of the ob gene. Expression of leptin in fully fed animals reflects adipocyte size and body-fat mass. Leptin signals the status of body energy stores to the brain, where signals emanate to regulate food intake and whole-body energy expenditure. The leptin gene was identified in the leptindeficient, obese ob/ob mouse by positional cloning techniques. Recently, leptin has been cloned in domestic species including pigs, cattle, and chickens. The receptor for OB has been identified in mouse, human and rat. In mouse, the mature receptor is a 1142 aa residue, type I (extracellular N-terminal) transmembrane protein with a predicted molecular weight of 81 kDa. The molecule shows 817 aa residues in its extracellular segment, 21 aa residues in its transmembrane domain, and 302 aa residues in its cytoplasmic tail. Mouse, human and rat OB receptors are all virtually identical in length, with the mouse extracellular and cytoplasmic segments exhibiting 77% and 72% aa identity with their human counterparts. The OB receptor is described as being a gp130 analog. The leptin receptor has at least five splice variants; the long form of the receptor is primarily expressed in the hypothalamus and is thought to be the predominant signaling isoform. Leptin receptors are members of the cytokine family of receptors and signal via janus-activated kinases(JAK)/signal transducers and activators of transcription (STAT) and mitogen-activated protein kinase (MAPK) pathways. Mutations in the leptin or leptin receptor genes results in morbid obesity, infertility, and insulin resistance in rodents and humans. Leptin receptors are expressed in most tissues, and in vitro evidence suggests that leptin may have direct effects on some tissues such as adipose tissue, the adrenal cortex, and the pancreatic beta-cell. Leptin is thought to influence whole-body glucose homeostasis and insulin action.

Database

Research Area

REFERENCE

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SDS
PBS.pdf
Package List Price ($)
$ 399