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Leptin antibody

Orthogonal Overexpression
Anti-Leptin antibody used in Western Blot (WB). GTX109204
Anti-Leptin antibody used in IHC (Paraffin sections) (IHC-P). GTX109204
Anti-Leptin antibody used in IHC (Paraffin sections) (IHC-P). GTX109204
Anti-Leptin antibody used in Western Blot (WB). GTX109204
Anti-Leptin antibody used in Western Blot (WB). GTX109204

Cat. No. GTX109204

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Leptin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

MCF-7 , MDA-MB-231 , DDDDK-tagged LEP-transfected 293T

Predict Reactivity

Rat, Rabbit, Bovine, Cat, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Leptin Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885271

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

leptin , LEPD , OB , OBS

Cellular Localization

Secreted

Background

This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Leptin antibody used in Western Blot (WB). GTX109204

GTX109204 WB Image

Orthogonal

MCF-7 whole cell and membrane extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Leptin antibody (GTX109204) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Leptin antibody used in IHC (Paraffin sections) (IHC-P). GTX109204

GTX109204 IHC-P Image

Leptin antibody detects Leptin protein at secreted on human lung papillary adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung papillary adenocarcinoma.
Leptin antibody (GTX109204) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Leptin antibody used in IHC (Paraffin sections) (IHC-P). GTX109204

GTX109204 IHC-P Image

Leptin antibody detects Leptin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse adrenal gland.
Leptin stained by Leptin antibody (GTX109204) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Leptin antibody used in Western Blot (WB). GTX109204

GTX109204 WB Image

Leptin antibody detects Leptin protein by Western blot analysis.
A. 30 μg MCF-7 whole cell lysate/extract
B. 30 μg MDA-MB-231 whole cell lysate/extract
12 % SDS-PAGE
Leptin antibody (GTX109204) dilution: 1:1000

Anti-Leptin antibody used in Western Blot (WB). GTX109204

GTX109204 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Leptin antibody (GTX109204) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Corsetti G et al. Nutrients 2022; 14 (14) Essential Amino Acids-Rich Diet Decreased Adipose Tissue Storage in Adult Mice: A Preliminary Histopathological Study.
Application : IHC-P
Reactivity : Mouse
Schendel SA et al. Aesthet Surg J 2017; (Epub) Autologous Adipose-Derived Tissue Matrix Part I: Biologic Characteristics.
Application : WB
Reactivity : Human
Chen WH et al. Am J Sports Med 2016; (Epub) Functional Recovery in Osteoarthritic Chondrocytes Through Hyaluronic Acid and Platelet-Rich Plasma-Inhibited Infrapatellar Fat Pad Adipocytes.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169