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Lipocalin-2 antibody, Internal

Anti-Lipocalin-2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89572
Anti-Lipocalin-2 antibody, Internal used in Western Blot (WB). GTX89572

Cat. No. GTX89572

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all Lipocalin-2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.01-0.03μg/ml
IHC-P 2-3μg/ml

Note :

IHC-P
Human Spleen shows staining of cytoplasm in mostly binucleated cells, and of nucleoli in some of the non-dividing cells.
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89572-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-AILREDKDPQKMY, from the internal region of the protein sequence according to NP_005555.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lipocalin 2 , 24p3 , MSFI , NGAL , p25

Cellular Localization

Secreted

Background

This gene encodes a protein that belongs to the lipocalin family. Members of this family transport small hydrophobic molecules such as lipids, steroid hormones and retinoids. The protein encoded by this gene is a neutrophil gelatinase-associated lipocalin and plays a role in innate immunity by limiting bacterial growth as a result of sequestering iron-containing siderophores. The presence of this protein in blood and urine is an early biomarker of acute kidney injury. This protein is thought to be be involved in multiple cellular processes, including maintenance of skin homeostasis, and suppression of invasiveness and metastasis. Mice lacking this gene are more susceptible to bacterial infection than wild type mice. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-Lipocalin-2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89572

GTX89572 IHC-P Image

IHC-P analysis of human spleen using GTX89572 Lipocalin-2 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

Anti-Lipocalin-2 antibody, Internal used in Western Blot (WB). GTX89572

GTX89572 WB Image

WB analysis of human kidney lysate using GTX89572 Lipocalin-2 antibody, Internal.
Dilution : 0.03μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399