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Liprin alpha 1 antibody [N1N2], N-term

GTX115098_WB_18121410_748.jpg
GTX115098_40247_WB_M_18121410_278.jpg
GTX115098_40247_20141017_WB_18121410_463.jpg
GTX115098_40247_20180131_ICC_IF_R_18121410_779.jpg
GTX115098_40247_20150717_IP_18121410_566.jpg

Cat No. GTX115098

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IP 1:100-1:500
IHC Assay dependent
Not tested in other applications.

Calculated MW

136 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , JurKat , Neuro2A

Predict Reactivity

Zebrafish, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

0.98mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Liprin alpha 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ41337 antibody, FLJ42630 antibody, FLJ43474 antibody, LIP.1 antibody, LIP1 antibody, LIPRIN antibody, MGC26800 antibody, PPFIA1 antibody, LAR-interacting protein 1 antibody, PTPRF-interacting protein alpha-1 antibody, Liprin-alpha1 antibody, liprin-alpha-1 antibody, protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-1 antibody, LIP-1 antibody, protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1 antibody

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. This protein binds to the intracellular membrane-distal phosphatase domain of tyrosine phosphatase LAR, and appears to localize LAR to cell focal adhesions. This interaction may regulate the disassembly of focal adhesion and thus help orchestrate cell-matrix interactions. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX115098_WB_18121410_748.jpg

GTX115098 WB Image

Sample (30 ug of whole cell lysate)
A: JurKat
5% SDS PAGE
GTX115098 diluted at 1:10000

GTX115098_40247_WB_M_18121410_278.jpg

GTX115098 WB Image

Liprin alpha 1 antibody [N1N2], N-term detects PPFIA1 protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
5 % SDS-PAGE
Liprin alpha 1 antibody [N1N2], N-term (GTX115098) dilution: 1:1000

GTX115098_40247_20141017_WB_18121410_463.jpg

GTX115098 WB Image

Liprin alpha 1 antibody detects Liprin alpha 1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Liprin alpha 1 antibody (GTX115098) diluted by 1:1000.

GTX115098_40247_20180131_ICC_IF_R_18121410_779.jpg

GTX115098 ICC/IF Image

Liprin alpha 1 antibody [N1N2], N-term detects Liprin alpha 1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Liprin alpha 1 stained by Liprin alpha 1 antibody [N1N2], N-term (GTX115098) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX115098_40247_20150717_IP_18121410_566.jpg

GTX115098 IP Image

Immunoprecipitation of Liprin alpha 1 protein from A431 whole cell extracts using 5 μg of Liprin alpha 1 antibody [N1N2], N-term (GTX115098).
Western blot analysis was performed using Liprin alpha 1 antibody [N1N2], N-term (GTX115098).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Reference
SDS
Glycerol.pdf
Thimerosal.pdf