APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:1000 |
| 1:50 |
| 1:100 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 1% BSA, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.36 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human recombinant protein of human BIRC7 (NP_071444) produced in HEK293T cell.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
baculoviral IAP repeat containing 7 , KIAP , LIVIN , ML-IAP , MLIAP , RNF50
Cellular Localization
Nucleus,Cytoplasm,Golgi apparatus
Background
Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, inhibits caspase-3 and proteolytic activation of pro-caspase-9. Isoform 1 blocks staurosporine-induced apoptosis. Isoform 2 blocks etoposide-induced apoptosis.
Database
Research Area
DATA IMAGES
![Anti-Livin antibody [1D12] used in Flow cytometry (FACS). GTX84823](/templates/default/js/lightbox/images/loading.gif "Anti-Livin antibody [1D12] used in Flow cytometry (FACS). GTX84823")
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GTX84823 FACS Image
FACS analysis of HeLa cells using GTX84823 Livin antibody [1D12].
Red : Primary antibody
Blue : Negative control antibody
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GTX84823 FACS Image
FACS analysis of Jurkat cells using GTX84823 Livin antibody [1D12].
Red : Primary antibody
Blue : Negative control antibody
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GTX84823 WB Image
WB analysis of various samples using GTX84823 Livin antibody [1D12].
Loading : 10 ug per lane
Dilution : 1:200
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GTX84823 WB Image
WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84823 Livin antibody [1D12].
Loading : 10 ug per lane
Dilution : 1:200
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GTX84823 WB Image
WB analysis of HEK293T cells transfected with Livin plasmid (Right) or empty vector (Left) for 48 hrs using GTX84823 Livin antibody [1D12].
Loading : 5 ug per lane
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GTX84823 IHC-P Image
IHC-P analysis of liver carcinoma tissue using GTX84823 Livin antibody [1D12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
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REFERENCE
There are currently no references for Livin antibody [1D12] (GTX84823). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Livin antibody [1D12] (GTX84823). Be the first to share your experience with this product.
![Anti-Livin antibody [1D12] used in Flow cytometry (FACS). GTX84823](/upload/website/prouct_img/small/GTX84823/GTX84823_561_FACS_19021902_247.jpg "Anti-Livin antibody [1D12] used in Flow cytometry (FACS). GTX84823")
![Anti-Livin antibody [1D12] used in Flow cytometry (FACS). GTX84823](/upload/website/prouct_img/small/GTX84823/GTX84823_562_FACS_19021902_855.jpg "Anti-Livin antibody [1D12] used in Flow cytometry (FACS). GTX84823")
![Anti-Livin antibody [1D12] used in Western Blot (WB). GTX84823](/upload/website/prouct_img/small/GTX84823/GTX84823_3573_WB_19021902_570.jpg "Anti-Livin antibody [1D12] used in Western Blot (WB). GTX84823")
![Anti-Livin antibody [1D12] used in Western Blot (WB). GTX84823](/upload/website/prouct_img/small/GTX84823/GTX84823_4263_WB_19021902_748.jpg "Anti-Livin antibody [1D12] used in Western Blot (WB). GTX84823")
![Anti-Livin antibody [1D12] used in Western Blot (WB). GTX84823](/upload/website/prouct_img/small/GTX84823/GTX84823_4647_WB_18122101_497.jpg "Anti-Livin antibody [1D12] used in Western Blot (WB). GTX84823")
![Anti-Livin antibody [1D12] used in IHC (Paraffin sections) (IHC-P). GTX84823](/upload/website/prouct_img/small/GTX84823/GTX84823_3144_IHC-P_18122101_421.jpg "Anti-Livin antibody [1D12] used in IHC (Paraffin sections) (IHC-P). GTX84823")
![Anti-Livin antibody [1D12] used in Flow cytometry (FACS). GTX84823](/upload/website/prouct_img/normal/GTX84823/GTX84823_561_FACS_19021902_247.jpg "Anti-Livin antibody [1D12] used in Flow cytometry (FACS). GTX84823")
![Anti-Livin antibody [1D12] used in Flow cytometry (FACS). GTX84823](/upload/website/prouct_img/normal/GTX84823/GTX84823_562_FACS_19021902_855.jpg "Anti-Livin antibody [1D12] used in Flow cytometry (FACS). GTX84823")
![Anti-Livin antibody [1D12] used in Western Blot (WB). GTX84823](/upload/website/prouct_img/normal/GTX84823/GTX84823_3573_WB_19021902_570.jpg "Anti-Livin antibody [1D12] used in Western Blot (WB). GTX84823")
![Anti-Livin antibody [1D12] used in Western Blot (WB). GTX84823](/upload/website/prouct_img/normal/GTX84823/GTX84823_4263_WB_19021902_748.jpg "Anti-Livin antibody [1D12] used in Western Blot (WB). GTX84823")
![Anti-Livin antibody [1D12] used in Western Blot (WB). GTX84823](/upload/website/prouct_img/normal/GTX84823/GTX84823_4647_WB_18122101_497.jpg "Anti-Livin antibody [1D12] used in Western Blot (WB). GTX84823")
![Anti-Livin antibody [1D12] used in IHC (Paraffin sections) (IHC-P). GTX84823](/upload/website/prouct_img/normal/GTX84823/GTX84823_3144_IHC-P_18122101_421.jpg "Anti-Livin antibody [1D12] used in IHC (Paraffin sections) (IHC-P). GTX84823")