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Livin antibody [1D12]

Overexpression
Anti-Livin antibody [1D12] used in Flow cytometry (FACS). GTX84823
Anti-Livin antibody [1D12] used in Flow cytometry (FACS). GTX84823
Anti-Livin antibody [1D12] used in Western Blot (WB). GTX84823
Anti-Livin antibody [1D12] used in Western Blot (WB). GTX84823
Anti-Livin antibody [1D12] used in IHC (Paraffin sections) (IHC-P). GTX84823
Anti-Livin antibody [1D12] used in Western Blot (WB). GTX84823

Cat. No. GTX84823

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1D12

Isotype

 IgG1

Application

 WB, IHC-P, FACS

Reactivity

 Human, Mouse
Package
100 μl ($519)
See all Livin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human BIRC7 (NP_071444) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

baculoviral IAP repeat containing 7 , KIAP , LIVIN , ML-IAP , MLIAP , RNF50

Cellular Localization

Nucleus,Cytoplasm,Golgi apparatus

Background

Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, inhibits caspase-3 and proteolytic activation of pro-caspase-9. Isoform 1 blocks staurosporine-induced apoptosis. Isoform 2 blocks etoposide-induced apoptosis.

Database

Research Area

DATA IMAGES

Anti-Livin antibody [1D12] used in Flow cytometry (FACS). GTX84823

GTX84823 FACS Image

FACS analysis of HeLa cells using GTX84823 Livin antibody [1D12].
Red : Primary antibody
Blue : Negative control antibody

Anti-Livin antibody [1D12] used in Flow cytometry (FACS). GTX84823

GTX84823 FACS Image

FACS analysis of Jurkat cells using GTX84823 Livin antibody [1D12].
Red : Primary antibody
Blue : Negative control antibody

Anti-Livin antibody [1D12] used in Western Blot (WB). GTX84823

GTX84823 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84823 Livin antibody [1D12].
Loading : 10 ug per lane
Dilution : 1:200

Anti-Livin antibody [1D12] used in Western Blot (WB). GTX84823

GTX84823 WB Image

WB analysis of various samples using GTX84823 Livin antibody [1D12].
Loading : 10 ug per lane
Dilution : 1:200

Anti-Livin antibody [1D12] used in IHC (Paraffin sections) (IHC-P). GTX84823

GTX84823 IHC-P Image

IHC-P analysis of liver carcinoma tissue using GTX84823 Livin antibody [1D12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-Livin antibody [1D12] used in Western Blot (WB). GTX84823

GTX84823 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Livin plasmid (Right) or empty vector (Left) for 48 hrs using GTX84823 Livin antibody [1D12].
Loading : 5 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519