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Livin antibody, C-term

Anti-Livin antibody, C-term used in Western Blot (WB). GTX89665
Anti-Livin antibody, C-term used in Western Blot (WB). GTX89665

Cat No. GTX89665

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89665-PEP).

Product Note

This antibody is expected to recognise both reported isoforms (as represented by NP_647478.1 and NP_071444.1)

PROPERTIES

Form

Liquid

Buffer

TBS pH7.3, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-RAPVRSRVRT, from the C Terminus of the protein sequence according to NP_647478.1; NP_071444.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

baculoviral IAP repeat containing 7 , KIAP , LIVIN , ML-IAP , MLIAP , RNF50

Cellular Localization

Nucleus , Cytoplasm , Golgi apparatus

Background

This gene encodes a member of the inhibitor of apoptosis protein (IAP) family, and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Elevated levels of the encoded protein may be associated with cancer progression and play a role in chemotherapy sensitivity. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-Livin antibody, C-term used in Western Blot (WB). GTX89665

GTX89665 WB Image

WB analysis of Daudi (A), Jurkat (B) and K562 (C) lysates using GTX89665 Livin antibody, C-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer

Anti-Livin antibody, C-term used in Western Blot (WB). GTX89665

GTX89665 WB Image

WB analysis of Molt-4 cell lysate using GTX89665 Livin antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 369