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Livin antibody

Anti-Livin antibody used in IHC (Paraffin sections) (IHC-P). GTX114132
Anti-Livin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114132
Anti-Livin antibody used in Western Blot (WB). GTX114132

Cat. No. GTX114132

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Livin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

293T , A431 , HepG2

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Livin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037353

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

baculoviral IAP repeat containing 7 , KIAP , LIVIN , ML-IAP , MLIAP , RNF50

Cellular Localization

Nucleus , Cytoplasm

Background

The protein encoded by this gene is a member of the family of inhibitor of apoptosis proteins (IAP) and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Two transcript variants encoding different isoforms have been found for this gene. The two isoforms have different antiapoptotic properties, with isoform alpha protecting cells from apoptosis induced by staurosporine and isoform b protecting cells from apoptosis induced by etoposide. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Livin antibody used in IHC (Paraffin sections) (IHC-P). GTX114132

GTX114132 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using ML-IAP(GTX114132) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Livin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114132

GTX114132 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ML-IAP(GTX114132) antibody at 1:200 dilution.

Anti-Livin antibody used in Western Blot (WB). GTX114132

GTX114132 WB Image

Livin antibody detects BIRC7 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
12 % SDS-PAGE
Livin antibody (GTX114132) dilution: 1:500

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169