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Livin antibody

Anti-Livin antibody used in Western Blot (WB). GTX31663
Anti-Livin antibody used in IHC (Paraffin sections) (IHC-P). GTX31663
Anti-Livin antibody used in IHC (Paraffin sections) (IHC-P). GTX31663

Cat No. GTX31663

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

Raji Cell Lysate(GTX27908) , Human Small Intestine Tissue Lysate(GTX27923) , Human Small Intestine Tissue Slide

Specificity/Sensitivity

A lower but much weaker band at 30 kDa was detected in Raji cell lysate, which may represent the short form of Livin.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Livin antibody was raised against a 17 amino acid peptide near the carboxy terminus of human Livin.The immunogen is located within the last 50 amino acids of Livin.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

baculoviral IAP repeat containing 7 , KIAP , LIVIN , ML-IAP , MLIAP , RNF50

Cellular Localization

Nucleus,Cytoplasm,Golgi apparatus

Background

The protein encoded by this gene is a member of the family of inhibitor of apoptosis proteins (IAP) and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Two transcript variants encoding different isoforms have been found for this gene. The two isoforms have different antiapoptotic properties, with isoform alpha protecting cells from apoptosis induced by staurosporine and isoform b protecting cells from apoptosis induced by etoposide. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Livin antibody used in Western Blot (WB). GTX31663

GTX31663 WB Image

WB analysis of Raji cell lysate using GTX31663 Livin antibody.
Working concentration : 0.5 μg/ml

Anti-Livin antibody used in IHC (Paraffin sections) (IHC-P). GTX31663

GTX31663 IHC-P Image

IHC-P analysis of human small intestine tissue using GTX31663 Livin antibody.
Working concentration : 5 μg/ml

Anti-Livin antibody used in IHC (Paraffin sections) (IHC-P). GTX31663

GTX31663 IHC-P Image

IHC-P analysis of human small intestine tissue using GTX31663 Livin antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339