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Livin antibody

Anti-Livin antibody used in Western Blot (WB). GTX74451

Cat No. GTX74451

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Human
Package
50 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-1μg/ml
ELISA 1/4,000

Note :

WB
This antibody detects bands of approximately 30 & 35kDa in MOLT4 cell lysates. A minimum incubation time of 1 hour is recommended with this antibody.

Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes an epitope within the C-terminal region of human Livin. This antibody is expected to recognize both isoforms of the molecule.

PROPERTIES

Form

Liquid

Buffer

Tris buffered saline, 0.02% Sodium Azide, 0.5% BSA

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence C-RAPVRSRVRT corresponding to the C-terminal region of LIVIN (UniProt: Q96CA5).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Baculoviral Iap Repeat Containing 7 , Kiap , Livin , Ml-Iap , Mliap , Rnf50 , Birc7

Background

The protein encoded by this gene is a member of the family of inhibitor of apoptosis proteins (IAP) and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Two transcript variants encoding different isoforms have been found for this gene. The two isoforms have different antiapoptotic properties, with isoform alpha protecting cells from apoptosis induced by staurosporine and isoform b protecting cells from apoptosis induced by etoposide. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Livin antibody used in Western Blot (WB). GTX74451

GTX74451 WB Image

WB analysis of MOLT4 cell lysate using GTX74451 Livin antibody.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 319