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Loricrin antibody [N1], N-term

Orthogonal
Anti-Loricrin antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116013
Anti-Loricrin antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116013
Anti-Loricrin antibody [N1], N-term used in Western Blot (WB). GTX116013
Anti-Loricrin antibody [N1], N-term used in Western Blot (WB). GTX116013

Cat. No. GTX116013

Reference 11

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Loricrin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Loricrin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037360

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

loricrin

Cellular Localization

Cytoplasm , Nucleus , nucleoplasm

Background

This gene encodes loricrin, a major protein component of the cornified cell envelope found in terminally differentiated epidermal cells. Mutations in this gene are associated with Vohwinkel's syndrome and progressive symmetric erythrokeratoderma, both inherited skin diseases. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Loricrin antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116013

GTX116013 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Hep3B, using Loricrin(GTX116013) antibody at 1:500 dilution.

Anti-Loricrin antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116013

GTX116013 IHC-P Image

Loricrin antibody [N1], N-term detects LOR protein at cytosol on HBL438 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL438 xenograft.
Loricrin antibody [N1], N-term (GTX116013) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Loricrin antibody [N1], N-term used in Western Blot (WB). GTX116013

GTX116013 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Loricrin antibody [N1], N-term (GTX116013) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Loricrin antibody [N1], N-term used in Western Blot (WB). GTX116013

GTX116013 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Loricrin antibody [N1], N-term (GTX116013) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed expression level is based on the publication: PMID: 12366693

REFERENCE

Application Reference

M?ller L et al. iScience 2024; 27 (9) : 110762 A feedback loop between plakophilin 4 and YAP signaling regulates keratinocyte differentiation.
Oh JS et al. Nutrients 2021; 13 (9) Lithospermum erythrorhizon Alleviates Atopic Dermatitis-like Skin Lesions by Restoring Immune Balance and Skin Barrier Function in 2.4-Dinitrochlorobenzene-Induced NC/Nga Mice.
Application : WB
Roy RR et al. Eur J Oral Sci 2021; 129 (1) : e12760 Contribution of transglutaminases and their substrate proteins to the formation of cornified cell envelope in oral mucosal epithelium.
Application : IHC-P
Kim SH et al. Nutrients 2020; 12 (1) Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice Through Modulating Immune Balance and Skin Barrier Function.
Application : WB
Wang C et al. Exp Dermatol 2019; (Epub) MiR-744-3p regulates keratinocyte proliferation and differentiation via targeting KLLN in psoriasis.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
M?ller L et al. iScience 2024; 27 (9):110762 A feedback loop between plakophilin 4 and YAP signaling regulates keratinocyte differentiation.
Oh JS et al. Nutrients 2021; 13 (9) Lithospermum erythrorhizon Alleviates Atopic Dermatitis-like Skin Lesions by Restoring Immune Balance and Skin Barrier Function in 2.4-Dinitrochlorobenzene-Induced NC/Nga Mice.
Application : WB
Roy RR et al. Eur J Oral Sci 2021; 129 (1):e12760 Contribution of transglutaminases and their substrate proteins to the formation of cornified cell envelope in oral mucosal epithelium.
Application : IHC-P
Kim SH et al. Nutrients 2020; 12 (1) Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice Through Modulating Immune Balance and Skin Barrier Function.
Application : WB
Wang C et al. Exp Dermatol 2019; (Epub) MiR-744-3p regulates keratinocyte proliferation and differentiation via targeting KLLN in psoriasis.
Application : WB
Reactivity : Human
Wang S et al. Med Sci (Paris) 2018; 34 Focus issue F1:94-98 Knock-down of filaggrin influences the mitogen-activated protein kinases signaling pathway in normal human epidermal keratinocytes.
Application : WB
Reactivity : Human
Shimada K et al. J Int Med Res 2018;:3.00E+14 Ectopic transglutaminase 1 and 3 expression accelerating keratinization in oral lichen planus.
Application : IHC-P
Reactivity : Human
Wang T et al. SpringerLink 2018; (Epub) The Role of miR-205 During Skin Wound Reepithelialization.
Application : IHC-P
Qian L et al. Cytokine 2017; 96:114-122 LncRNA expression profile of ΔNp63α in cervical squamous cancers and its suppressive effects on LIF expression.
Wang T et al. Biochim Biophys Acta 2016; 1862 (8):1443-1452 Downregulation of miR-205 in migrating epithelial tongue facilitates skin wound re-epithelialization by derepressing ITGA5.
Dang NN et al. Braz J Med Biol Res 2015; 48 (1):39-45 Filaggrin silencing by shRNA directly impairs the skin barrier function of normal human epidermal keratinocytes and then induces an immune response.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169