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Loricrin antibody

Anti-Loricrin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127943
Anti-Loricrin antibody used in Western Blot (WB). GTX127943

Cat No. GTX127943

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:2000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Loricrin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

loricrin

Cellular Localization

Cytoplasm , Nucleus , nucleoplasm

Background

This gene encodes loricrin, a major protein component of the cornified cell envelope found in terminally differentiated epidermal cells. Mutations in this gene are associated with Vohwinkel's syndrome and progressive symmetric erythrokeratoderma, both inherited skin diseases. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Loricrin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127943

GTX127943 ICC/IF Image

Loricrin antibody detects Loricrin protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Loricrin protein stained by Loricrin antibody (GTX127943) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Loricrin antibody used in Western Blot (WB). GTX127943

GTX127943 WB Image

Loricrin antibody detects loricrin protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
12 % SDS-PAGE
Loricrin antibody (GTX127943) dilution: 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169