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Ly6g antibody [RB6-8C5] (PE)

Anti-Ly6g antibody [RB6-8C5] (PE) used in Flow cytometry (FACS). GTX42973

Cat. No. GTX42973

Reference 11

Host

 Rat

Clonality

 Monoclonal

Clone Name

 RB6-8C5

Isotype

 IgG2b

Application

 IHC-P, FACS

Reactivity

 Mouse
Package
100 μg ($429)
See all Ly6g products
PRODUCT

Summary

The RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P Assay dependent
FACS Assay dependent
Not tested in other applications.

Product Note

This antibody reacts predominantly with the Ly-6G protein but weaker reactivity with the Ly-6C protein has been reported (Fleming et al. 1993).

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Normal murine bone marrow cells.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Phycoerythrin (PE)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lymphocyte antigen 6 complex, locus G , Gr-1 , Gr1 , Ly-6G

Database

Research Area

DATA IMAGES

Anti-Ly6g antibody [RB6-8C5] (PE) used in Flow cytometry (FACS). GTX42973

GTX42973 FACS Image

FACS analysis of C57Bl/6 bone marrow cells using GTX42973 Ly6g antibody [RB6-8C5] (PE).
Solid line : Primary antibody
Dashed line : PE rat IgG2b isotype control
Antibody amount : 0.06 μg

REFERENCE

Clone Reference

Yuanjia Zhu et al. Sci Rep 2022; 12 (1):10028 A novel photosynthetic biologic topical gel for enhanced localized hyperoxygenation augments wound healing in peripheral artery disease.
Yamamoto Y et al. Life Sci Alliance 2022; 5 (10) Neutrophils delay repair process in Wallerian degeneration by releasing NETs outside the parenchyma.
Xu Q et al. Front Pharmacol 2021; 12:655372 Emodin Alleviates Severe Acute Pancreatitis-Associated Acute Lung Injury by Inhibiting the Cold-Inducible RNA-Binding Protein (CIRP)-Mediated Activation of the NLRP3/IL-1β/CXCL1 Signaling.
Liang H et al. PeerJ 2021; 9:e10644 Perfluorooctane sulfonate exerts inflammatory bowel disease-like intestinal injury in rats.
Wu F et al. Inflammation 2019; (Epub) Cellular Inflammatory Response of the Spleen After Acute Spinal Cord Injury in Rat.
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REVIEW

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Clone Reference
Yuanjia Zhu et al. Sci Rep 2022; 12 (1):10028 A novel photosynthetic biologic topical gel for enhanced localized hyperoxygenation augments wound healing in peripheral artery disease.
Yamamoto Y et al. Life Sci Alliance 2022; 5 (10) Neutrophils delay repair process in Wallerian degeneration by releasing NETs outside the parenchyma.
Xu Q et al. Front Pharmacol 2021; 12:655372 Emodin Alleviates Severe Acute Pancreatitis-Associated Acute Lung Injury by Inhibiting the Cold-Inducible RNA-Binding Protein (CIRP)-Mediated Activation of the NLRP3/IL-1β/CXCL1 Signaling.
Liang H et al. PeerJ 2021; 9:e10644 Perfluorooctane sulfonate exerts inflammatory bowel disease-like intestinal injury in rats.
Wu F et al. Inflammation 2019; (Epub) Cellular Inflammatory Response of the Spleen After Acute Spinal Cord Injury in Rat.
Pollenus E et al. Immunol Lett. 2019; 212:30-36 Limitations of neutrophil depletion by anti-Ly6G antibodies in two heterogenic immunological models.
Application : FACS
Reactivity : Mouse
Calcinotto A et al. Nature 2018; 559 (7714):363-369 IL-23 secreted by myeloid cells drives castration-resistant prostate cancer.
Xu D et al. Front Cell Infect Microbiol. 2018; 8:157 Gr-1+ Cells Other Than Ly6G+ Neutrophils Limit Virus Replication and Promote Myocardial Inflammation and Fibrosis Following Coxsackievirus B3 Infection of Mice.
Application : FACS
Reactivity : Mouse
Muller I et al. Circ Heart Fail 2017; 10 (11) Pathogenic Role of the Damage-Associated Molecular Patterns S100A8 and S100A9 in Coxsackievirus B3-Induced Myocarditis.
Muller I et al. PLoS One 2017; 12 (8):e0182643 CX3CR1 knockout aggravates Coxsackievirus B3-induced myocarditis.
Jinjin Xu et al. Nat Commun. 2016; The REGγ-proteasome forms a regulatory circuit with IκB? and NFκB in experimental colitis.
Application : IHC-P, FACS
Reactivity : Mouse
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 429