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Lysyl-tRNA synthetase antibody

Anti-Lysyl-tRNA synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX102113
Anti-Lysyl-tRNA synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX102113
Anti-Lysyl-tRNA synthetase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102113
Anti-Lysyl-tRNA synthetase antibody used in Western Blot (WB). GTX102113

Cat. No. GTX102113

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Lysyl-tRNA synthetase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lysyl-tRNA synthetase. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_1241002

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysyl-tRNA synthetase , CMTRIB , DFNB89 , KARS1 , KARS2 , KRS

Cellular Localization

Cytoplasm

Background

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Lysyl-tRNA synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX102113

GTX102113 IHC-P Image

Lysyl-tRNA synthetase antibody detects Lysyl-tRNA synthetase protein at cytosol on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Lysyl-tRNA synthetase antibody (GTX102113) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Lysyl-tRNA synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX102113

GTX102113 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using Lysyl-tRNA synthetase(GTX102113) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Lysyl-tRNA synthetase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102113

GTX102113 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Lysyl-tRNA synthetase(GTX102113) antibody at 1:500 dilution.

Anti-Lysyl-tRNA synthetase antibody used in Western Blot (WB). GTX102113

GTX102113 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX102113 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Halpert MM et al. FASEB J 2020; 34 (6) : 8082-8101 MHC class I and II peptide homology regulates the cellular immune response.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169