GeneTex
United States (US)

Lysyl-tRNA synthetase antibody

GTX102113_WB_19011506_994.jpg
GTX102113_39609_IHC_M_19011506_372.jpg
GTX102113_40254_IFA_19011506_240.jpg
GTX102113_39609_IHC_19011506_634.jpg

Cat No. GTX102113

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lysyl-tRNA synthetase. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CMTRIB antibody, KARS2 antibody, KIAA0070 antibody, KRS antibody, KARS antibody, lysRS antibody, lysyl-tRNA synthetase antibody, lysine tRNA ligase antibody

Cellular Localization

Cytoplasm

Background

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102113_WB_19011506_994.jpg

GTX102113 WB Image

Sample (30 µg of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX102113 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102113_39609_IHC_M_19011506_372.jpg

GTX102113 IHC-P Image

Lysyl-tRNA synthetase antibody detects Lysyl-tRNA synthetase protein at cytosol on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Lysyl-tRNA synthetase antibody (GTX102113) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102113_40254_IFA_19011506_240.jpg

GTX102113 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Lysyl-tRNA synthetase(GTX102113) antibody at 1:500 dilution.

GTX102113_39609_IHC_19011506_634.jpg

GTX102113 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using Lysyl-tRNA synthetase(GTX102113) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169