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Lysyl-tRNA synthetase antibody

Anti-Lysyl-tRNA synthetase antibody used in Western Blot (WB). GTX102179
Anti-Lysyl-tRNA synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX102179

Cat No. GTX102179

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Lysyl-tRNA synthetase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

lysyl-tRNA synthetase , CMTRIB , DFNB89 , KARS1 , KARS2 , KRS

Cellular Localization

Cytoplasm

Background

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Lysyl-tRNA synthetase antibody used in Western Blot (WB). GTX102179

GTX102179 WB Image

Sample (30 ug of whole cell lysate)
A: A549 7.5% SDS PAGE
GTX102179 diluted at 1:1000

Anti-Lysyl-tRNA synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX102179

GTX102179 IHC-P Image

Lysyl-tRNA synthetase antibody detects KARS protein at cytoplasm on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
Lysyl-tRNA synthetase antibody (GTX102179) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169