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Lysyl-tRNA synthetase antibody

Anti-Lysyl-tRNA synthetase antibody used in Western Blot (WB). GTX66204
Anti-Lysyl-tRNA synthetase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66204

Cat. No. GTX66204

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 298-597 of human KARS (NP_005539.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysyl-tRNA synthetase , CMTRIB , DFNB89 , KARS1 , KARS2 , KRS

Cellular Localization

Cytoplasm,Nucleus,Cell membrane,Secreted

Background

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Lysyl-tRNA synthetase antibody used in Western Blot (WB). GTX66204

GTX66204 WB Image

WB analysis of various sample lysates using GTX66204 Lysyl-tRNA synthetase antibody.
Dilution : 1:3000
Loading : 25μg per lane

Anti-Lysyl-tRNA synthetase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66204

GTX66204 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX66204 Lysyl-tRNA synthetase antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

There are currently no references for Lysyl-tRNA synthetase antibody (GTX66204). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399