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M-CSF antibody, Internal

Anti-M-CSF antibody, Internal used in Flow cytometry (FACS). GTX81735
Anti-M-CSF antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81735
Anti-M-CSF antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81735
Anti-M-CSF antibody, Internal used in Western Blot (WB). GTX81735

Cat. No. GTX81735

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all M-CSF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:10-1:50
IHC-P 1:10-1:50
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 230-257 amino acids from the Central region of human M-CSF.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

colony stimulating factor 1 , CSF-1 , MCSF

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-M-CSF antibody, Internal used in Flow cytometry (FACS). GTX81735

GTX81735 FACS Image

FACS analysis of 293 cells using GTX81735 M-CSF antibody, Internal.
Green : primary antibody
Blue : negative control

Anti-M-CSF antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81735

GTX81735 IHC-P Image

IHC-P analysis of human kidney tissue using GTX81735 M-CSF antibody, Internal.

Anti-M-CSF antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81735

GTX81735 ICC/IF Image

ICC/IF analysis of MDA-MB231 cells using GTX81735 M-CSF antibody, Internal.
Green : M-CSF
Blue : DAPI

Anti-M-CSF antibody, Internal used in Western Blot (WB). GTX81735

GTX81735 WB Image

WB analysis of human placenta tissue lysate using GTX81735 M-CSF antibody, Internal.
Loading : 35ug per lane
Dilution : 1:1000

REFERENCE

Application Reference

Lin NY et al. Mol Cancer Res 2023; 21 (7) : 664-674 Truncation of GalNAc-type O-glycans Suppresses CD44-mediated Osteoclastogenesis and Bone Metastasis in Breast Cancer.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399