GeneTex
United States (US)

M-CSF antibody

Cat No. GTX10675

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ELISA, Neut
Reactivity Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.2 μg/ml
ELISA 0.5-1.0 μg/ml
Neut 0.015-0.035 μg/ml
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Specificity/Sensitivity

By immunoblotting and ELISA, the antibody shows 5% cross-reactivity with recombinant human M-CSF.
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Mouse

Immunogen

recombinant mouse M-CSF expressed in Escherichia coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Colony Stimulating Factor 1 (Macrophage),C87615,Csfm,Mcsf,Op,Csf1

Background

Four distinct colony-stimulating factors (CSFs) that promote survival, proliferation and differentiation of bone marrow precursor cells have been well characterized: granulocyte macrophage CSF (GMCSF), granulocyte CSF (GCSF), macrophage CSF (MCSF), and Interleukin-3 (IL-3, Multi CSF). Both GMCSF and IL-3 are multipotential growth factors, stimulating proliferation of progenitor cells from more than one hematopoietic lineage. In contrast, GCSF and MCSF are lineage restricted hematopoietic growth factors, stimulating final mitotic divisions and the terminal cellular maturation of the partially differentiated hematopoietic progenitors.Macrophage CSF, also known as CSF1, is produced by monocytes, fibroblasts and endothelial cells. It stimulates the formation of macrophage colonies, enhances antibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages, and inhibits bone resorption by osetoclasts. Natural human MCSF is a dimeric glycoprotein of 70-90 kD molecular weight, existing in multiple glycosylation forms. It binds to a 165 kD glycoprotein of the receptor tyrosine kinase subclass III, a family that includes the receptors for platelet derived growth factor (PDGF) and stem cell factor (SCF).

Database

Research Area

SDS
PBS.pdf
Package List Price ($)
$ 319