GeneTex
United States (US)

M-CSF antibody

Cat No. GTX29693

Host Rabbit
Clonality Polyclonal
Application WB, ELISA, Neut
Reactivity Human
APPLICATION

Application Note

Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hM-CSF (7.0 ng/ml), a concentration of 0.02 - 0.03 μg/ml of this antibody is required. ELISA:To detect hM-CSF by direct ELISA (using 100μl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hM-CSF. Western Blot:To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
PROPERTIES

Form

Liquid

Buffer

PBS, pH 7.4, no preservative, sterile filtered

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Highly pure (>98%) recombinant hM-CSF (human M-CSF)

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CSF1 Macrophage Colony Stimulating Factor 1

Cellular Localization

Secreted

Background

Macrophage Colony Stimulating Factor (M-CSF) is produced by various cell types including fibroblasts, macrophages and endothelial cells. This colony stimulation factor is involved in both the development and function of monocytes/macrophages.

Research Area

Package List Price ($)
$ 339