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M/NEI antibody

Anti-M/NEI antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54692
Anti-M/NEI antibody used in Western Blot (WB). GTX54692

Cat No. GTX54692

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:20 - 1:100
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 100-379 of human SERPINB1 (NP_109591.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

serpin family B member 1 , EI , ELANH2 , HEL-S-27 , HEL57 , LEI , M/NEI , MNEI , PI-2 , PI2

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the serpin family of proteinase inhibitors. Members of this family maintain homeostasis by neutralizing overexpressed proteinase activity through their function as suicide substrates. This protein inhibits the neutrophil-derived proteinases neutrophil elastase, cathepsin G, and proteinase-3 and thus protects tissues from damage at inflammatory sites. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Database

Research Area

DATA IMAGES

Anti-M/NEI antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54692

GTX54692 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX54692 M/NEI antibody.
Blue : DAPI

Anti-M/NEI antibody used in Western Blot (WB). GTX54692

GTX54692 WB Image

WB analysis of various sample lysates using GTX54692 M/NEI antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf