APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 2 μg/mL |
| 2.5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Mouse Liver Tissue Lysate(GTX27935) , Human Liver Tissue Slide , Human Liver Tissue Lysate
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
MACC1 antibody was raised against a 17 amino acid synthetic peptide near the center of human MACC1.The immunogen is located within amino acids 430 - 480 of MACC1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
MET transcriptional regulator MACC1 , 7A5 , SH3BP4L
Cellular Localization
Cytoplasm,Nucleus
Background
Metastasis associated in colon cancer 1 (MACC1) is a key regulator of the hepatocyte growth factor (HGF)-HGF receptor (MET) pathway, which is involved in cellular growth, epithelial-mesenchymal transition, angiogenesis, cell motility, invasiveness, and metastasis. MACC1 protein consists of four domains: ZU5, SH3, and two C-terminal death domains (DD). Expression of MACC1 was found significantly upregulated in malignant tissues (colon cancer of all stages as well as liver and lung metastases) compared to normal tissues or adenomas. MACC1 represents an early and crucial prognostic indicator for colon cancer metastasis that is independent of age, sex, tumor infiltration, nodal status, and lymph vessel invasion. Besides its involvement in signal transduction with the MET receptor, MACC1 also links MET signaling and apoptosis. MACC1 may also be an important therapeutic target for colorectal cancer treatment. At least two isoforms of MACC1 are known to exist.
Database
Research Area
DATA IMAGES
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GTX85281 WB Image
WB analysis of human liver tissue lysate using GTX85281 MACC1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml
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GTX85281 IHC-P Image
IHC-P analysis of human liver tissue using GTX85281 MACC1 antibody.
Working concentration : 2.5 μg/ml
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GTX85281 IHC-P Image
IHC-P analysis of human liver tissue using GTX85281 MACC1 antibody.
Working concentration : 20 μg/ml
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GTX85281 WB Image
WB analysis of mouse liver tissue lysate using GTX85281 MACC1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml
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REFERENCE
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REVIEW
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