*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A431 , HeLa , HepG2 , A375
Mouse, Rat(>80% identity)
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1mg/ml(Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human MAD1. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
MAD1 antibody, PIG9 antibody, TP53I9 antibody, TXBP181 antibody, MAD1L1 antibody, mitotic arrest deficient 1-like protein 1 antibody, mitotic spindle assembly checkpoint protein MAD1 antibody, mitotic checkpoint MAD1 protein homolog antibody, tax-binding protein 181 antibody, mitotic-arrest deficient 1, yeast, homolog-like 1 antibody, tumor protein p53 inducible protein 9 antibody, MAD1-like protein 1 antibody, MAD1 mitotic arrest deficient-like 1 (yeast) antibody
Nucleus , Kinetochore
MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
GTX105079 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using MAD1(GTX105079) antibody at 1:500 dilution.
GTX105079 IHC-P Image
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using MAD1(GTX105079) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX105079 WB Image
MAD1 antibody detects MAD1L1 protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
E. 30 µg A375 whole cell lysate/extract
MAD1 antibody (GTX105079) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX105079 IP Image
Immunoprecipitation of MAD1 protein from HeLa whole cell extracts using 5 μg of MAD1 antibody (GTX105079).
Western blot analysis was performed using MAD1 antibody (GTX105079).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.