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MAD1 antibody

Anti-MAD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105079
Anti-MAD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105079
Anti-MAD1 antibody used in Western Blot (WB). GTX105079
Anti-MAD1 antibody used in Immunoprecipitation (IP). GTX105079

Cat No. GTX105079

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375

Predict Reactivity

Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

mitotic arrest deficient 1 like 1 , MAD1 , PIG9 , TP53I9 , TXBP181

Cellular Localization

Nucleus , Kinetochore

Background

MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-MAD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105079

GTX105079 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using MAD1(GTX105079) antibody at 1:500 dilution.

Anti-MAD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105079

GTX105079 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using MAD1(GTX105079) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MAD1 antibody used in Western Blot (WB). GTX105079

GTX105079 WB Image

MAD1 antibody detects MAD1L1 protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
E. 30 µg A375 whole cell lysate/extract
7.5% SDS-PAGE
MAD1 antibody (GTX105079) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MAD1 antibody used in Immunoprecipitation (IP). GTX105079

GTX105079 IP Image

Immunoprecipitation of MAD1 protein from HeLa whole cell extracts using 5 μg of MAD1 antibody (GTX105079).
Western blot analysis was performed using MAD1 antibody (GTX105079).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169