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MAD1 antibody

Anti-MAD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32710
Anti-MAD1 antibody used in Western Blot (WB). GTX32710
Anti-MAD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32710
Anti-MAD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32710

Cat. No. GTX32710

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all MAD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 360-700 of human MAD1L1 (NP_001013859.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitotic arrest deficient 1 like 1 , MAD1 , PIG9 , TP53I9 , TXBP181

Cellular Localization

Nucleus

Background

MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Database

Research Area

DATA IMAGES

Anti-MAD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32710

GTX32710 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX32710 MAD1 antibody.
Dilution : 1:100

Anti-MAD1 antibody used in Western Blot (WB). GTX32710

GTX32710 WB Image

WB analysis of various sample lysates using GTX32710 MAD1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-MAD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32710

GTX32710 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX32710 MAD1 antibody.
Dilution : 1:100

Anti-MAD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32710

GTX32710 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32710 MAD1 antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399