GeneTex
United States (US)

MAD2L1 antibody [17D10]

GTX10691_20160330_WB_19011506_631.jpg

Cat No. GTX10691

Host Mouse
Clonality Monoclonal
Clone Name 17D10
Isotype IgG1
Application WB, IP, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:200 - 1:2000
IP 1:100
ELISA 1:5000 - 1:20000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

HeLa whole cell lysate
PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This protein A purified monoclonal antibody was produced by repeated immunizations with full-length recombinant human MAD2L1 protein.

Purification

Protein A purified
Purified from supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mitotic Arrest Deficient 2 Like 1,Hsmad2,Mad2,Mad2L1

Cellular Localization

Nuclear

Background

MAD2L1 (also called mitotic spindle assembly checkpoint protein, MAD2A, MAD2-like 1 and HsMAD2) is a component of the mitotic spindle assembly checkpoint monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. MAD2L1 inhibits the activity of the anaphase-promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. This protein has a nuclear localization.

Database

Research Area

DATA IMAGES
GTX10691_20160330_WB_19011506_631.jpg

GTX10691 WB Image

Western blot using GeneTex Mab anti-MAD2L1 antibody (GTX10691) shows detection of a band at ~24 kDa (arrowhead) corresponding to MAD2L1 present in a HeLa whole cell lysate (lane 1). Approximately 75 µg of lysate was separated by 4-20% TG SDS-PAGE. After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1:200. The membrane was washed and reacted with a 1:5,000 dilution of IRDye™800 conjugated Sh-a-Mouse IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.

Package List Price ($)
$ 319