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MAD2L1 antibody [C2C3], C-term

KO/KD
Anti-MAD2L1 antibody [C2C3], C-term used in Western Blot (WB). GTX104680
Anti-MAD2L1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104680
Anti-MAD2L1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104680
Anti-MAD2L1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104680
Anti-MAD2L1 antibody [C2C3], C-term used in Western Blot (WB). GTX104680
Anti-MAD2L1 antibody [C2C3], C-term used in Western Blot (WB). GTX104680
Anti-MAD2L1 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX104680
Anti-MAD2L1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104680

Cat. No. GTX104680

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all MAD2L1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

HepG2 , Molt-4 , Raji , 293T

Predict Reactivity

Mouse, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MAD2L1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241033

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitotic arrest deficient 2 like 1 , HSMAD2 , MAD2

Background

MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MAD2L1 antibody [C2C3], C-term used in Western Blot (WB). GTX104680

GTX104680 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term (GTX104680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MAD2L1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104680

GTX104680 ICC/IF Image

Immunofluorescence analysis of human osteosarcoma cell line U2OS, using MAD2L1(GTX104680) antibody at 1:1000 dilution.

Anti-MAD2L1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104680

GTX104680 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using Mad2L1(GTX104680) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MAD2L1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104680

GTX104680 IHC-P Image

MAD2L1 antibody [C2C3], C-term detects MAD2L1 protein at cytoplasm and nucleus in human A549 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded human A549 xenograft .
MAD2L1 antibody [C2C3], C-term (GTX104680) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MAD2L1 antibody [C2C3], C-term used in Western Blot (WB). GTX104680

GTX104680 WB Image

Sample (30 μg whole cell lysate)
A:Hep G2 (GTX27900)
B:MOLT4 (GTX27912)
C:Raji (GTX27908)
12% SDS PAGE
GTX104680 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MAD2L1 antibody [C2C3], C-term used in Western Blot (WB). GTX104680

GTX104680 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term (GTX104680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MAD2L1 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX104680

GTX104680 IP Image

Immunoprecipitation of MAD2L1 protein from 293T whole cell extracts using 5 μg of MAD2L1 antibody [C2C3], C-term (GTX104680).
Western blot analysis was performed using MAD2L1 antibody [C2C3], C-term (GTX104680).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-MAD2L1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104680

GTX104680 IHC-P Image

MAD2L1 antibody [C2C3], C-term detects MAD2L1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
MAD2L1 antibody [C2C3], C-term (GTX104680) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Wu YH et al. PLoS Pathog 2021; 17 (3) : e1008866 Aerobic glycolysis supports hepatitis B virus protein synthesis through interaction between viral surface antigen and pyruvate kinase isoform M2.
Application : WB
Reactivity : Human
Jerabkova K et al. FASEB J 2020; (Epub) Deubiquitylase UCHL3 regulates bi-orientation and segregation of chromosomes during mitosis.
Chen Y et al. Oncol Rep 2012; 27 (2) : 487-91 The spindle checkpoint protein MAD1 regulates the expression of E-cadherin and prevents cell migration.
Application : IP
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169