GeneTex
United States (US)

MAD2L1 antibody [C2C3], C-term

KO/KD
GTX104680_39638_20160818_WB_shRNA_watermark_19011506_951.jpg
GTX104680_IHC_19011506_472.jpg
GTX104680_IF1_19011506_535.jpg
GTX104680_39638_20151227_IHC-P_19011506_802.jpg
GTX104680_WB_19011506_624.jpg
GTX104680_39638_IHC_R_19011506_804.jpg
GTX104680_39638_20150407_IP_19011506_830.jpg

Cat No. GTX104680

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

293T , NCIN87 , U2OS , HepG2 , Molt4 , Raji

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MAD2L1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mitotic Arrest Deficient 2 Like 1,Hsmad2,Mad2,Mad2L1

Background

MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104680_39638_20160818_WB_shRNA_watermark_19011506_951.jpg

GTX104680 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term (GTX104680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104680_IHC_19011506_472.jpg

GTX104680 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using Mad2L1(GTX104680) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104680_IF1_19011506_535.jpg

GTX104680 ICC/IF Image

Immunofluorescence analysis of human osteosarcoma cell line U2OS, using MAD2L1(GTX104680) antibody at 1:1000 dilution.

GTX104680_39638_20151227_IHC-P_19011506_802.jpg

GTX104680 IHC-P Image

MAD2L1 antibody [C2C3], C-term detects MAD2L1 protein at cytoplasm and nucleus in human A549 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded human A549 xenograft .
MAD2L1 antibody [C2C3], C-term (GTX104680) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104680_WB_19011506_624.jpg

GTX104680 WB Image

Sample (30 µg whole cell lysate)
A:Hep G2 (GTX27900)
B:MOLT4 (GTX27912)
C:Raji (GTX27908)
12% SDS PAGE
GTX104680 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104680_39638_IHC_R_19011506_804.jpg

GTX104680 IHC-P Image

MAD2L1 antibody [C2C3], C-term detects MAD2L1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
MAD2L1 antibody [C2C3], C-term (GTX104680) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104680_39638_20150407_IP_19011506_830.jpg

GTX104680 IP Image

Immunoprecipitation of MAD2L1 protein from 293T whole cell extracts using 5 μg of MAD2L1 antibody [C2C3], C-term (GTX104680).
Western blot analysis was performed using MAD2L1 antibody [C2C3], C-term (GTX104680).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169