MAD2L1 antibody [C2C3], C-term

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term (GTX104680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term (GTX104680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

MAD2L1 antibody [C2C3], C-term detects MAD2L1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
MAD2L1 antibody [C2C3], C-term (GTX104680) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunoprecipitation of MAD2L1 protein from 293T whole cell extracts using 5 μg of MAD2L1 antibody [C2C3], C-term (GTX104680).
Western blot analysis was performed using MAD2L1 antibody [C2C3], C-term (GTX104680).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Sample (30 μg whole cell lysate)
A:Hep G2 (GTX27900)
B:MOLT4 (GTX27912)
C:Raji (GTX27908)
12% SDS PAGE
GTX104680 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

MAD2L1 antibody [C2C3], C-term detects MAD2L1 protein at cytoplasm and nucleus in human A549 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded human A549 xenograft .
MAD2L1 antibody [C2C3], C-term (GTX104680) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using Mad2L1(GTX104680) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of human osteosarcoma cell line U2OS, using MAD2L1(GTX104680) antibody at 1:1000 dilution.