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MAD2L2 antibody

Anti-MAD2L2 antibody used in IHC (Paraffin sections) (IHC-P). GTX23630
Anti-MAD2L2 antibody used in Western Blot (WB). GTX23630

Cat. No. GTX23630

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
50 μg ($399)
See all MAD2L2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:500 - 1:2000
ELISA 1:7500 - 1:30000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate, 0.15M NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: TLTRQDLNFGQV, corresponding to amino acids 3-14 of Human Mad2L2.

Purification

Purified by antigen-affinity chromatography.
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitotic arrest deficient 2 like 2 , FANCV , MAD2B , POLZ2 , REV7

Cellular Localization

Nucleus,Cytoplasm

Background

MAD2L2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L2 is a homolog of MAD2L1.

Database

Research Area

DATA IMAGES

Anti-MAD2L2 antibody used in IHC (Paraffin sections) (IHC-P). GTX23630

GTX23630 IHC-P Image

GeneTex Affinity Purified anti-MAD2L2 antibody (GTX23630) shows strong nuclear and cytoplasmic staining of tumor cells in cancerous human kidney tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei.

Anti-MAD2L2 antibody used in Western Blot (WB). GTX23630

GTX23630 WB Image

Affinity Purified Rabbit anti-MAD2L2 was used at a 1:500 dilution to detect human MAD2L2 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. This antibody clearly detects a ~20 kDa band corresponding to human MAD2L2 (predicted molecular weight is 24 kDa). Approximately 20 μg of each lysate was loaded on a 10% SDS-PAGE. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 30 sec.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399