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MADD antibody

Anti-MADD antibody used in Western Blot (WB). GTX22043
Anti-MADD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22043
Anti-MADD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22043

Cat No. GTX22043

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($409)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:250-1:500
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

183 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide corresponding to aa 1570-1588 of human MADD. This peptide sequence is identical to that of DENN and differs by one amino acid with rat GDP/GTP exchange protein RAB3-GEP.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DENN , IG20 , MADD , MAP kinase activating death domain , RAB3GEP

Cellular Localization

Membrane,Cytoplasm

Background

Tumor necrosis factor alpha (TNF-alpha) is a signaling molecule that interacts with one of two receptors on cells targeted for apoptosis. The apoptotic signal is transduced inside these cells by cytoplasmic adaptor proteins. The protein encoded by this gene is a death domain-containing adaptor protein that interacts with the death domain of TNF-alpha receptor 1 to activate mitogen-activated protein kinase (MAPK) and propagate the apoptotic signal. It is membrane-bound and expressed at a higher level in neoplastic cells than in normal cells. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MADD antibody used in Western Blot (WB). GTX22043

GTX22043 WB Image

Western blot analysis of MADD in whole cell lysates from the indicated cell lines with Anti-MADD (GTX22043) at 1:250 dilution.

Anti-MADD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22043

GTX22043 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22043 MADD antibody.
Dilution : 20 μg/ml

Anti-MADD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22043

GTX22043 ICC/IF Image

ICC/IF analysis of human spleen cells using GTX22043 MADD antibody.
Dilution : 10 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 409