GeneTex
United States (US)

MAEA antibody

GTX115394_40302_IFA_18121410_902.jpg
GTX115394_40302_WB_18121410_485.jpg

Cat No. GTX115394

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

HeLa , HepG2 , HCT116

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAEA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Macrophage Erythroblast Attacher,Emlp,Emp,Gid9,Hlc-10,Pig5,Maea

Cellular Localization

Nucleus matrix , Cell membrane , Cytoplasm , cytoskeleton

Background

This gene product mediates the attachment of erythroblasts to macrophages. This attachment promotes terminal maturation and enucleation of erythroblasts, presumably by suppressing apoptosis. This protein is an integral membrane protein with the N-terminus on the extracellular side and the C-terminus on the cytoplasmic side of the cell. Two immunologically related isoforms of erythroblast macrophage protein with apparent molecular weights of 33 kD and 36 kD were detected in macrophage membranes; this gene encodes the larger isoform. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX115394_40302_IFA_18121410_902.jpg

GTX115394 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using MAEA(GTX115394) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX115394_40302_WB_18121410_485.jpg

GTX115394 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
10% SDS PAGE
GTX115394 diluted at 1:5000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169